Alexanian Arshak R
Neuroscience Research Labs, Dept. of Neurosurgery, Medical College of Wisconsin, VAMC, 5000 W. National Ave. 151, Milwaukee, WI 53295, USA.
Exp Cell Res. 2005 Nov 1;310(2):383-91. doi: 10.1016/j.yexcr.2005.08.015. Epub 2005 Oct 5.
Several recent reports suggest that there is far more plasticity that previously believed in the developmental potential of bone-marrow-derived cells (BMCs) that can be induced by extracellular developmental signals of other lineages whose nature is still largely unknown. In this study, we demonstrate that bone-marrow-derived mesenchymal stem cells (MSCs) co-cultured with mouse proliferating or fixed (by paraformaldehyde or methanol) neural stem cells (NSCs) generate neural stem cell-like cells with a higher expression of Sox-2 and nestin when grown in NS-A medium supplemented with N2, NSC conditioned medium (NSCcm) and bFGF. These neurally induced MSCs eventually differentiate into beta-III-tubulin and GFAP expressing cells with neuronal and glial morphology when grown an additional week in Neurobasal/B27 without bFGF. We conclude that juxtacrine interaction between NSCs and MSCs combined with soluble factors released from NSCs are important for generation of neural-like cells from bone-marrow-derived adherent MSCs.
最近的几份报告表明，骨髓源性细胞（BMCs）的发育潜力具有比以前认为的更大的可塑性，其可由其他谱系的细胞外发育信号诱导，而这些信号的性质在很大程度上仍然未知。在本研究中，我们证明，与小鼠增殖或固定（用多聚甲醛或甲醇）的神经干细胞（NSCs）共培养的骨髓源性间充质干细胞（MSCs），在补充有N2、NSC条件培养基（NSCcm）和bFGF的NS-A培养基中生长时，会产生具有较高Sox-2和巢蛋白表达的神经干细胞样细胞。当在不含bFGF的Neurobasal/B27中再培养一周时，这些经神经诱导的MSCs最终分化为具有神经元和神经胶质形态的表达β-III-微管蛋白和GFAP的细胞。我们得出结论，NSCs和MSCs之间的旁分泌相互作用以及NSCs释放的可溶性因子对于从骨髓源性贴壁MSCs生成神经样细胞很重要。
