N-[6-(乙二氧基)己基]生物素酰胺的合成：一种用于标记肼修饰生物分子的生物素醛前体。

Synthesis of N-[6-(ethylenedioxy)hexyl]biotinamide: a biotinyl aldehyde precursor for labeling hydrazine-modified biomolecules.

作者信息

Musso G F, Scarlato G R, Ghosh S S

机构信息

SISKA Diagnostics, Inc., San Diego, California.

出版信息

Bioconjug Chem. 1992 Jan-Feb;3(1):88-90. doi: 10.1021/bc00013a015.

DOI:10.1021/bc00013a015

PMID:1616955

Abstract

Synthetic approaches for obtaining biotinyl aldehydes are described. While such aldehydes have limited shelflife, the acetal derivative, N-[6-(ethylenedioxy)hexyl]biotinamide, IX, was found to be indefinitely stable upon storage at -20 degrees C. Mild acid hydrolysis conveniently unmasks the aldehyde, which can then be used to label hydrazine-tagged biomolecules.

摘要

本文描述了获取生物素醛的合成方法。虽然此类醛的保质期有限，但发现缩醛衍生物N-[6-(乙二氧基)己基]生物素酰胺（IX）在-20℃储存时可无限期稳定保存。温和的酸水解可方便地使醛基暴露，然后可用于标记带有肼标记的生物分子。