Kedzierska Karolina, Bober Joanna, Ciechanowski Kazimierz, Gołembiewska Edyta, Kwiatkowska Ewa, Noceń Iwona, Dołegowska Barbara, Dutkiewicz Grazyna, Chlubek Dariusz
Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University, Szczecin, Poland.
Biol Trace Elem Res. 2005 Oct;107(1):21-32. doi: 10.1385/BTER:107:1:021.
The aim of the study was to verify the hypothesis if copper could influence the activity of sodium-transporting systems in erythrocyte membrane that could be related to essential hypertension. The examined group of patients consisted of 15 men with hypertension. The control group was 11 healthy male volunteers. The Na+/H+ exchanger (NHE) activity in erythrocytes was determined according to Orlov et al. The activity of transporting systems (ATP-Na+/K+; co-Na+/K+/Cl-; ex-Na+/Li+; free Na+ and K+ outflow [Na+, K+-outflow]) was determined according to Garay's method. The concentration of copper in plasma was assessed using atomic absorption spectrometry. The activity of ATP-Na+/K+ (micromol/L red blood cells [RBCs]/h) in hypertensive patients was 2231.5 +/- 657.6 vs 1750.5 +/- 291 in the control (p < 0.05), the activity of co-Na+/K+/Cl- (micromol/L RBCs/h) in hypertensives was 171.3 +/- 77.9 vs 150.7 +/- 53.9 in the control (NS). Na+-outflow (micromol/L RBCs/h) in hypertensives was 118.3 +/- 51.6 vs 113.3 +/- 24.4 in the control (NS). The K+-outflow (micromol/L RBCs/h) in hypertensives was 1361.7 +/- 545.4 vs 1035.6 +/- 188.3 in the control (NS). The activity of ex-Na+/Li+ (micromol/L RBCs/h) in hypertensive patients was 266.1 +/- 76.1 vs 204.1 +/- 71.6 in the control (p < 0.05). NHE activity (mmol/L RBCs/h) in hypertensives was 9.7 +/- 2.96 vs 7.7 +/- 1.33 in the control (p < 0.05). In hypertensive patients, negative correlation was found between the activity of Na+/K+/Cl- co-transport and plasma copper concentration (Rs = -0.579, p < 0.05) and between the activity of ex-Na+/Li+ and plasma copper concentration (Rs = -0.508, p < 0.05). Plasma copper concentration significantly influences the activity of sodium transporting systems in erythrocyte membrane. Copper supplementation could be expected to provide therapeutic benefits for hypertensive patients.
该研究的目的是验证铜是否会影响红细胞膜中与原发性高血压相关的钠转运系统的活性这一假设。被检查的患者组由15名高血压男性组成。对照组是11名健康男性志愿者。红细胞中的Na+/H+交换体（NHE）活性根据奥尔洛夫等人的方法测定。转运系统（ATP-Na+/K+；协同Na+/K+/Cl-；外排Na+/Li+；游离Na+和K+外流[Na+，K+-外流]）的活性根据加雷的方法测定。血浆中的铜浓度使用原子吸收光谱法评估。高血压患者中ATP-Na+/K+的活性（微摩尔/升红细胞[RBCs]/小时）为2231.5±657.6，而对照组为1750.5±291（p<0.05）；高血压患者中协同Na+/K+/Cl-的活性（微摩尔/升RBCs/小时）为171.3±77.9，而对照组为150.7±53.9（无显著性差异）。高血压患者的Na+外流（微摩尔/升RBCs/小时）为118.3±51.6，而对照组为113.3±24.4（无显著性差异）。高血压患者的K+外流（微摩尔/升RBCs/小时）为1361.7±545.4，而对照组为1035.6±188.3（无显著性差异）。高血压患者中外排Na+/Li+的活性（微摩尔/升RBCs/小时）为266.1±76.1，而对照组为204.
