Lijnen P, Fenyvesi A
Department of Pathophysiology, University of Leuven, Belgium.
Methods Find Exp Clin Pharmacol. 1994 Jan-Feb;16(1):37-47.
The relationship between erythrocyte cation transport systems, membrane and plasma lipids, plasma prorenin and microalbuminuria was examined in normal men and patients with insulin dependent diabetes mellitus (IDDM). Different measurements of erythrocyte transport systems were obtained in patients with IDDM and in age- and weight-matched healthy men: Na+/Li(+)-countertransport activity, Na+/K(+)-cotransport activity, Na+/K(+)-ATPase pump activity and the ground membrane permeability for Na+ and K+ as well as the intraerythrocyte Na+, K+ and Mg2+ concentration. Plasma prorenin, cholesterol, triglycerides, phospholipids, low and high density lipoprotein cholesterol and erythrocyte membrane cholesterol and phospholipids content were also obtained from the fasting subjects. The patients with IDDM had an elevated (p < 0.05 or less) erythrocyte Na+/Li(+)-countertransport activity, ground membrane leak for K+, intraerythrocyte K+ concentration, erythrocyte membrane cholesterol content, but a lower red blood cell phospholipids content. In single regression analysis the erythrocyte Na+/Li(+)-countertransport, Na+/K(+)-cotransport and Na+/K(+)-ATPase pump activity and ground membrane leak for Na+ and K+ were inversely related to the red cell membrane lipid content. The erythrocyte Na+/Li(+)-countertransport activity and K+ leak were also positively related to the plasma prorenin level and urinary microalbumin excretion. Our data in patients with IDDM show that an elevated erythrocyte membrane lipid content was accompanied by a lower erythrocyte Na+/Li(+)-countertransport, Na+/K(+)-cotransport or Na+/K(+)-ATPase pump activity. The elevated Na+/Li(+)-countertransport activity was also accompanied by a higher plasma prorenin level and microalbuminuria.
在正常男性和胰岛素依赖型糖尿病（IDDM）患者中，研究了红细胞阳离子转运系统、膜及血浆脂质、血浆肾素原与微量白蛋白尿之间的关系。对IDDM患者以及年龄和体重匹配的健康男性进行了不同的红细胞转运系统测量：钠/锂（Na⁺/Li⁺）逆向转运活性、钠/钾（Na⁺/K⁺）协同转运活性、钠/钾（Na⁺/K⁺）-ATP酶泵活性以及钠和钾的基础膜通透性，还有红细胞内钠、钾和镁离子浓度。还从空腹受试者中获取了血浆肾素原、胆固醇、甘油三酯、磷脂、低密度和高密度脂蛋白胆固醇以及红细胞膜胆固醇和磷脂含量。IDDM患者的红细胞钠/锂（Na⁺/Li⁺）逆向转运活性、钾的基础膜渗漏、红细胞内钾浓度、红细胞膜胆固醇含量升高（p<0.05或更低），但红细胞磷脂含量较低。在单因素回归分析中，红细胞钠/锂（Na⁺/Li⁺）逆向转运、钠/钾（Na⁺/K⁺）协同转运和钠/钾（Na⁺/K⁺）-ATP酶泵活性以及钠和钾的基础膜渗漏与红细胞膜脂质含量呈负相关。红细胞钠/锂（Na⁺/Li⁺）逆向转运活性和钾渗漏也与血浆肾素原水平和尿微量白蛋白排泄呈正相关。我们在IDDM患者中的数据表明，红细胞膜脂质含量升高伴随着红细胞钠/锂（Na⁺/Li⁺）逆向转运、钠/钾（Na⁺/K⁺）协同转运或钠/钾（Na⁺/K⁺）-ATP酶泵活性降低。钠/锂（Na⁺/Li⁺）逆向转运活性升高还伴随着血浆肾素原水平升高和微量白蛋白尿。
