Woodward S R, Sudweeks J, Teuscher C
Department of Microbiology, Brigham Young University, Provo, Utah 84602.
Mamm Genome. 1992;3(2):73-8. doi: 10.1007/BF00431249.
The random amplification of polymorphic DNA (RAPD) using primers of arbitrary nucleotide sequence has been extremely valuable in identifying heritable markers in a variety of systems. The present studies examined whether the RAPD technique can identify large numbers of polymorphisms that can be used to construct genetic maps in inbred strains of mice. By screening the inbred mouse strains C57BL/6J and DBA/2J with 481 random 10-mer oligonucleotide primers, we identified 95 polymorphisms and mapped 76 of these by use of the BXD series of recombinant inbred (RI) strains. The results clearly demonstrate that the RAPD technique allows for the identification of large numbers of DNA-based polymorphisms that distinguish these two inbred strains of mice, and that such markers can readily be used to construct molecular genetic linkage maps.
使用任意核苷酸序列引物进行的随机扩增多态性DNA（RAPD）技术，在鉴定各种系统中的可遗传标记方面具有极高价值。本研究探讨了RAPD技术能否识别大量可用于构建近交系小鼠遗传图谱的多态性。通过用481个随机的10聚体寡核苷酸引物筛选近交系小鼠C57BL/6J和DBA/2J，我们鉴定出95个多态性，并利用BXD系列重组近交（RI）系对其中76个进行了定位。结果清楚地表明，RAPD技术能够识别区分这两种近交系小鼠的大量基于DNA的多态性，并且这些标记可轻易用于构建分子遗传连锁图谱。
