Martínková J, Rýdlová I, Horák V
Charles University, Faculty of Medicine, Department of Pharmacology, Hradec Králové, Czechoslovakia.
Exp Toxicol Pathol. 1992 Apr;44(2):102-4. doi: 10.1016/S0940-2993(11)80196-X.
The model of biliary tract infection induced in rats given suspension of E. coli into the bile duct is described. To prevent leakage of microorganisms after the administration, a temporary ligation of the bile duct followed. Contemporary groups of sham-operated and control rats (given saline by intrabiliary injection) were compared to assess the significance of the changes. The effect of biliary infection was concentration dependent. If 0.1 ml of the concentration containing 10(2), 10(3) and 10(6) colony-forming units/ml was injected, the mortality of rats reached 8%, 57% and 65%, respectively within 24 h. Blood and bile cultures from all dead animals grew E. coli. To evaluate the effect of chronic biliary infection, the concentration of 10(2) colony-forming units/ml was used. Serum concentrations of total and conjugated bilirubin, cholesterol and creatinine, activities of S-alanine-aminotransferase, S-aspartate-aminotransferase, alkaline phosphatase, the count of leucocytes in blood, total body weight with weight of the liver were investigated on days 1, 4 and 12 after the treatment. The results showed: an increase in leucocytes (21 +/- 4.2 10(9)/l, p less than 0.02 vs control animals) on day 4, an augmentation of serum cholesterol on day 1, (2.1 +/- 0.9 mmol/l, p less than 0.02 vs control animals), the presence of E. coli in blood on day 1 and its persistence in the bile on days 1, 4 and 12. Except the bile, all of the other symptoms were reversible by day 12.
描述了将大肠杆菌悬液注入大鼠胆管诱导胆道感染的模型。为防止给药后微生物泄漏，随后对胆管进行临时结扎。将假手术组和对照组大鼠（经胆管内注射生理盐水）进行比较，以评估这些变化的意义。胆道感染的影响呈浓度依赖性。如果分别注射0.1 ml含10²、10³和10⁶菌落形成单位/ml浓度的菌液，大鼠在24小时内的死亡率分别达到8%、57%和65%。所有死亡动物的血液和胆汁培养均生长出大肠杆菌。为评估慢性胆道感染的影响，使用了10²菌落形成单位/ml的浓度。在治疗后第1、4和12天，检测血清总胆红素和结合胆红素、胆固醇和肌酐的浓度，S-丙氨酸转氨酶、S-天冬氨酸转氨酶、碱性磷酸酶的活性，血液白细胞计数，总体重以及肝脏重量。结果显示：第4天白细胞增多（21±4.2×10⁹/l，与对照动物相比p<0.02），第1天血清胆固醇升高（2.1±0.9 mmol/l，与对照动物相比p<0.02），第1天血液中出现大肠杆菌，且在第1、4和12天持续存在于胆汁中。除胆汁外，所有其他症状在第12天时均可逆转。
