Dy M, Kamoun P, Hamburger J
C R Seances Acad Sci D. 1979 Dec 17;289(16):1245-9.
An increased production of urea has been demonstrated during mixed cultures between allograft donor and recipient leukocytes. This phenomenon may also be observed during primary MLC (i.e, without previous allograft) but to a lesser degree. This increased production of urea during MLC results from an increase of arginase activity in spleen cell population(s) resulting in an increased arginine transformation into urea and ornithine, as demonstrated by the release of an amount of ornithine equivalent to that of urea in the culture supernatant.
在同种异体移植供体和受体白细胞的混合培养过程中，已证实尿素生成增加。在初次混合淋巴细胞培养（即无前移植）期间也可观察到这种现象，但程度较轻。混合淋巴细胞培养期间尿素生成增加是由于脾细胞群中精氨酸酶活性增加，导致精氨酸转化为尿素和鸟氨酸增加，这可通过培养上清液中释放的鸟氨酸量与尿素量相当来证明。
