Rogers C J, Hunter B E
Department of Neuroscience, University of Florida, College of Medicine, Gainesville 32610.
Brain Res Bull. 1992 Apr;28(4):587-92. doi: 10.1016/0361-9230(92)90107-9.
The effect of chronic ethanol exposure on inhibition in the rat hippocampal slice was investigated using paired-pulse stimulation techniques with stimulation in stratum radiatum or stratum oriens of CA1. Experimental animals were fed ethanol in a liquid diet for 20 weeks and were withdrawn for at least 8 weeks prior to electrophysiological recording. Prior ethanol treatment had no effect on basic input-output relationships for the extracellular population spike. Ethanol treatment significantly reduced the recurrent inhibition produced by antidromic stimulation in a manner dependent upon stimulus intensity. In addition, with orthodromic paired-pulse stimulation of either stratum radiatum or oriens, a trend toward an augmentation of the facilitation of population spike amplitude was observed, suggesting that feedforward inhibition may also be reduced. These results are similar to those found with treatments that reduce inhibition. Therefore, we conclude that chronic ethanol exposure produces an enduring disruption of inhibitory neuronal function in the rat hippocampus.
采用配对脉冲刺激技术，在大鼠海马切片的CA1区辐射层或原层进行刺激，研究慢性乙醇暴露对大鼠海马切片抑制作用的影响。实验动物通过液体饮食摄入乙醇20周，并在进行电生理记录前至少戒断8周。先前的乙醇处理对细胞外群体锋电位的基本输入-输出关系没有影响。乙醇处理以依赖刺激强度的方式显著降低了逆向刺激产生的回返抑制。此外，对辐射层或原层进行顺向配对脉冲刺激时，观察到群体锋电位幅度易化增强的趋势，这表明前馈抑制也可能降低。这些结果与使用减少抑制作用的处理所发现的结果相似。因此，我们得出结论，慢性乙醇暴露会对大鼠海马中的抑制性神经元功能产生持久的破坏。
