Yang Jun, Liu Wei, Yu Lei, Qiu Xiao-zhong, Jiao Pei-feng, Hu Lian-mei, Wu Lei, Piao Ying-jie, Zhong Shi-zhen, Qin Jian-qiang
Institute of Clinical Anatomy, Southern Medical University, Guangzhou 510515, China.
Di Yi Jun Yi Da Xue Xue Bao. 2005 Sep;25(9):1128-31, 1144.
To culture Schwann cells (SCs) and human hair keratins (HHKs) for artificial nerve bridge construction.
SCs were purified by primary culture and labeled with BrdU, which were then cultured with HHKs decorated by ECM. The artificial nerve bridge was implanted into the defect of sciatic nerve, beneath the skin, and in the skeletal muscles of SD rat, respectively. The morphology of the SCs cultured with HHKs was monitored by inverted microscope and evaluated by immunocytochemical staining. Growth of BrdU-labeled SCs in vivo was observed by immunocytochemical staining on paraffin sections.
In vitro cultured SCs were capable of adhering to HHKs and grew well four weeks after implantation. The HHK component in the artificial nerve bridge underwent degradation in the defect of the sciatic nerve, beneath the skin, and in the skeletal muscles of SD rat, and SC survival and proliferation were verified.
SCs can survive in three-dimensional culture with HHKs for construction of artificial nerve bridge to repair nerve defects.
培养雪旺细胞（SCs）和人发角蛋白（HHKs）用于构建人工神经桥。
通过原代培养纯化SCs并用BrdU标记，然后与经细胞外基质（ECM）修饰的HHKs共同培养。将人工神经桥分别植入SD大鼠坐骨神经缺损处、皮下及骨骼肌内。用倒置显微镜监测与HHKs共同培养的SCs的形态，并通过免疫细胞化学染色进行评估。通过石蜡切片上的免疫细胞化学染色观察体内BrdU标记的SCs的生长情况。
体外培养的SCs能够黏附于HHKs，植入四周后生长良好。人工神经桥中的HHK成分在SD大鼠坐骨神经缺损处、皮下及骨骼肌内发生降解，证实了SCs的存活和增殖。
SCs可与HHKs在三维培养中存活，用于构建人工神经桥以修复神经缺损。
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