[在大鼠模型中应用羊膜衍生物复合物卷与培养的自体雪旺细胞修复周围神经缺损]
[Repair of peripheral nerve defect by a scroll of amnion derivative compound with cultured autogenous Schwann cell in a rat model].
作者信息
Zhang Qi, Gu Xiao-ming, Yu Guang-yan, Mao Tian-qiu, Zheng Jing-chen, Tong Qing-ying
机构信息
Department of Stomatology, General Hospital of Chinese People's Armed Police Force, Beijing 100039, China.
出版信息
Zhonghua Kou Qiang Yi Xue Za Zhi. 2006 Feb;41(2):98-101.
OBJECTIVE
To test a nerve bridge substitute for peripheral nerve repair by tissue-engineering approach.
METHODS
An artificial nerve fabricated with a scroll of amnion derivative (ZQ membrane) and cultured autogenous Schwann cell was sutured to bridge sciatic nerve defect of 2.5 cm in length in rats. The specimens were assessed with tracking study, histology, electrophysiological technique, NF200, and synaptophysin-38 (SYP) immuno histochemical staining 3 months postoperatively.
RESULTS
The regenerated nerve sprouted 3 months after the operation. The regenerated nerve fibers were plentiful and could grow into the recipient nerve and target muscle's motor end plate (MEP) areas to reinnervate target muscle, and reconstruct function of nerve-muscle junction. Functional recovery could reach to 40%-60% of normal control. Nerve-muscle conduction velocity (N-MCV) arrived at 21.77 +/- 1.15 m/s.
CONCLUSIONS
A tissue engineering material fabricated with a scroll of ZQ membrane and cultured autologous Schwann cell may be a useful substitute for nerve repair.
目的
通过组织工程方法测试一种用于周围神经修复的神经桥替代物。
方法
将用羊膜衍生物（ZQ膜）卷和培养的自体雪旺细胞制成的人工神经缝合到大鼠2.5厘米长的坐骨神经缺损处。术后3个月，通过追踪研究、组织学、电生理技术、NF200和突触素-38（SYP）免疫组织化学染色对标本进行评估。
结果
术后3个月再生神经发芽。再生神经纤维丰富，可长入受体神经和靶肌肉的运动终板（MEP）区域，重新支配靶肌肉，并重建神经-肌肉接头功能。功能恢复可达正常对照的40%-60%。神经-肌肉传导速度（N-MCV）达到21.77±1.15米/秒。
结论
用ZQ膜卷和培养的自体雪旺细胞制成的组织工程材料可能是一种有用的神经修复替代物。
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