Paschinger Katharina, Fabini Gustáv, Schuster David, Rendić Dubravko, Wilson Iain B H
Department für Chemie, Universität für Bodenkultur, Wien, Austria.
Acta Biochim Pol. 2005;52(3):629-32. Epub 2005 Sep 15.
Carbohydrates are known as sources of immunological cross-reactivity of allergenic significance. In celery and in cypress pollen, the major allergens Api g 5 and Cup a 1 are recognised by antisera raised against anti-horseradish peroxidase and by patients' IgE which apparently bind carbohydrate epitopes; mass spectrometric analysis of the tryptic peptides and of their N-glycans showed the presence of oligosaccharides carrying both xylose and core alpha1,3-fucose residues. Core alpha1,3-fucose residues are also a feature of invertebrates: genetic and biochemical studies on the fruitfly Drosophila melanogaster, the parasitic trematode Schistosoma mansoni and the nematode worm Caenorhabditis elegans indicate that these organisms possess core alpha1,3-fucosyltransferases. Various experiments have shown that fucosyltransferases from both fly and worm are responsible in vivo and in vitro for the synthesis of N-glycans which cross-react with anti-horseradish peroxidase; thus, we can consider these enzymes as useful tools in generating standard compounds for testing cross-reactive carbohydrate epitopes of allergenic interest.
碳水化合物是具有变应原意义的免疫交叉反应性的来源。在芹菜和柏树花粉中，主要变应原Api g 5和Cup a 1可被抗辣根过氧化物酶产生的抗血清以及显然结合碳水化合物表位的患者IgE识别；对胰蛋白酶肽及其N-聚糖的质谱分析表明存在同时带有木糖和核心α1,3-岩藻糖残基的寡糖。核心α1,3-岩藻糖残基也是无脊椎动物的一个特征：对果蝇黑腹果蝇、寄生吸虫曼氏血吸虫和线虫秀丽隐杆线虫的遗传和生化研究表明，这些生物体拥有核心α1,3-岩藻糖基转移酶。各种实验表明，来自果蝇和线虫的岩藻糖基转移酶在体内和体外负责合成与抗辣根过氧化物酶发生交叉反应的N-聚糖；因此，我们可以将这些酶视为生成用于测试具有变应原意义的交叉反应性碳水化合物表位的标准化合物的有用工具。
