Piatek Rafał, Zalewska Beata, Bury Katarzyna, Kur Józef
Department of Microbiology, Gdańsk University of Technology, Poland.
Acta Biochim Pol. 2005;52(3):639-46.
The chaperone-usher system determines the biogenesis of surface-exposed adhesive structures responsible for virulence of many Gram-negative bacteria. Investigations of the last 20 years have resolved the mechanism of this pathway on a structural level for different species of pathogenic bacteria. The purpose of this review is to present the molecular mechanisms of the biogenesis of adhesive structures assembled via the chaperone-usher pathway. The obtained mechanistic data allow one to propose potential strategies of anti-bacterial action. Additionally, the specific properties of the polymeric adhesive structures (pili and fimbriae) of the chaperone-usher system allow their use as effective and safe recombinant vaccines carrying foreign epitopes in thousands of copies on bacterial cell surface.
伴侣蛋白-外膜蛋白转运系统决定了许多革兰氏阴性菌表面暴露的负责毒力的黏附结构的生物合成。过去20年的研究在结构层面解析了不同病原菌物种该途径的机制。本综述的目的是阐述通过伴侣蛋白-外膜蛋白转运途径组装的黏附结构生物合成的分子机制。所获得的机制数据使人们能够提出潜在的抗菌作用策略。此外，伴侣蛋白-外膜蛋白转运系统的聚合黏附结构（菌毛和纤毛）的特殊性质使其能够用作在细菌细胞表面携带数千个拷贝的外源表位的有效且安全的重组疫苗。
