Werneburg Glenn T, Thanassi David G
Department of Molecular Genetics and Microbiology.
EcoSal Plus. 2018 Mar;8(1). doi: 10.1128/ecosalplus.ESP-0007-2017.
Gram-negative bacteria assemble a variety of surface structures, including the hair-like organelles known as pili or fimbriae. Pili typically function in adhesion and mediate interactions with various surfaces, with other bacteria, and with other types of cells such as host cells. The chaperone/usher (CU) pathway assembles a widespread class of adhesive and virulence-associated pili. Pilus biogenesis by the CU pathway requires a dedicated periplasmic chaperone and integral outer membrane protein termed the usher, which forms a multifunctional assembly and secretion platform. This review addresses the molecular and biochemical aspects of the CU pathway in detail, focusing on the type 1 and P pili expressed by uropathogenic as model systems. We provide an overview of representative CU pili expressed by and , and conclude with a discussion of potential approaches to develop antivirulence therapeutics that interfere with pilus assembly or function.
革兰氏阴性菌会组装多种表面结构，包括被称为菌毛或纤毛的毛发状细胞器。菌毛通常在黏附中发挥作用，并介导与各种表面、其他细菌以及与其他类型细胞（如宿主细胞）的相互作用。伴侣/外膜蛋白途径（chaperone/usher，CU）组装了一类广泛存在的与黏附及毒力相关的菌毛。通过CU途径进行菌毛生物合成需要一种专门的周质伴侣蛋白和一种被称为外膜蛋白的整合外膜蛋白，后者形成一个多功能的组装和分泌平台。本综述详细阐述了CU途径的分子和生化方面，重点关注尿路致病性大肠杆菌表达的1型菌毛和P菌毛作为模型系统。我们概述了大肠杆菌和肺炎克雷伯菌表达的代表性CU菌毛，并以讨论开发干扰菌毛组装或功能的抗毒力疗法的潜在方法作为结尾。
