Veronesi M C, Faustini M, Villani M, Kindahl H, Galeati G, Battocchio M
Dipartimento di Scienze Cliniche Veterinarie, Sez. Clinica Ostetrica e Ginecologica Veterinaria, Milan, Italy.
J Vet Med A Physiol Pathol Clin Med. 2005 Oct;52(8):411-5. doi: 10.1111/j.1439-0442.2005.00748.x.
The aim of the present study was to highlight the effect of two different techniques of one embryo crushing on some hormonal changes. Ten twinning mares were submitted to the mobile or fixed manual crushing of one blastocyst within day 19 after the last mating. Blood sample was collected from 20 min before to 90 min, 24 and 72 h after the procedure was performed to analyse 15-ketodihydro-PGF(2alpha), cortisol and progesterone plasma concentrations. Singleton pregnancy diagnosis was checked 72 h after crushing and at term of pregnancy. Because the unwanted crushing of both embryos occurred in one mare during the attempt of manual separation of the twins, that mare was not included in the evaluation of crushing-induced hormonal changes. No significant differences in hormonal concentrations were observed after one embryo crushing and also when the effect of the mobile (n = 6) or fixed (n = 3) technique was specifically evaluated. When the effect of the two techniques on each post-crushing sampling time hormonal levels was analysed, only a higher cortisol level 30 min after the fixed compared with the mobile technique was observed. The crushing performed within 19 days of gestation does not induce significant changes in 15-ketodihydro-PGF(2alpha), cortisol or progesterone plasma concentrations. When the fixed technique was performed, only a temporary higher cortisol concentration was seen 30 min after crushing, suggesting that the fixed technique might be responsible for a slight level of stress for the mare.
本研究的目的是强调两种不同的单胚胎挤压技术对某些激素变化的影响。十匹怀有双胞胎的母马在最后一次交配后第19天内接受了对一个囊胚的手动移动或固定挤压。在操作前20分钟至操作后90分钟、24小时和72小时采集血样，以分析血浆中15-酮二氢-PGF(2α)、皮质醇和孕酮的浓度。在挤压后72小时和妊娠足月时检查单胎妊娠诊断情况。由于在尝试手动分离双胞胎的过程中，一匹母马出现了两个胚胎均被意外挤压的情况，该母马未被纳入挤压诱导激素变化的评估。在单胚胎挤压后，以及在专门评估移动技术（n = 6）或固定技术（n = 3）的效果时，均未观察到激素浓度有显著差异。当分析两种技术对每次挤压后采样时间激素水平的影响时，仅观察到与移动技术相比，固定技术在挤压后30分钟时皮质醇水平更高。在妊娠19天内进行的挤压不会引起血浆中15-酮二氢-PGF(2α)、皮质醇或孕酮浓度的显著变化。当采用固定技术时，仅在挤压后30分钟时观察到皮质醇浓度暂时升高，这表明固定技术可能会使母马产生轻微程度的应激。
