Lewinson D, Shurtz-Swirski R, Shenzer P, Wingender E, Mayer H, Silbermann M
Laboratory for Musculoskeletal Research, Rappaport Family Institute for Research in the Medical Sciences, Haifa, Israel.
Cell Tissue Res. 1992 May;268(2):257-66. doi: 10.1007/BF00318794.
This investigation presents the structural changes in condylar cartilage incubated in the presence of human parathyroid hormone (1-34) in an organ culture system for 6 to 12 days. Control cultures maintained their cartilaginous characteristics whereas human parathyroid hormone (1-34)-treated cultures revealed the following modifications: (1) The chondroprogenitor cell zone at the apical region of the explant underwent a substantial enlargement. The cells changed from a mesenchyme-like morphology into polygonal, glycogen-rich cells that were tightly attached to each other by a fibrillar intercellular matrix, but even by 12 days the apical region was comprised of healthy cells. (2) The mineralizing zone in the hypertrophic cartilage revealed a change in its cellular population. Hypertrophic chondrocytes were replaced by cells with amoeboid extensions and large numbers of secretory granules or vesicles. Based upon the above findings it appears that the chondroprogenitor cells that are initially stimulated to proliferate, are being suppressed from subsequent differentiation into chondroblasts; and that hypertrophic chondrocytes apparently undergo a dedifferentiation process followed by development into an as yet unknown cell population.
本研究展示了在器官培养系统中，人甲状旁腺激素（1-34）存在的情况下，髁突软骨培养6至12天的结构变化。对照培养物保持其软骨特征，而经人甲状旁腺激素（1-34）处理的培养物显示出以下改变：（1）外植体顶端区域的软骨祖细胞区显著扩大。细胞从间充质样形态转变为多边形、富含糖原的细胞，这些细胞通过纤维状细胞间基质彼此紧密相连，但即使到12天时，顶端区域仍由健康细胞组成。（2）肥大软骨中的矿化区显示其细胞群体发生了变化。肥大软骨细胞被具有变形虫样突起以及大量分泌颗粒或小泡的细胞所取代。基于上述发现，似乎最初被刺激增殖的软骨祖细胞，随后被抑制分化为成软骨细胞；并且肥大软骨细胞显然经历了去分化过程，随后发育为一个尚不清楚的细胞群体。
