Livne E, Weiss A, Silbermann M
Rappaport Family Institute for Research in the Medical Sciences, Technion-Israel Institute of Technology, Haifa.
Cell Differ. 1988 Mar;23(1-2):61-7. doi: 10.1016/0045-6039(88)90037-1.
The apical segments of the mandibular condylar cartilage of newborn ICR mice, containing the intact zones of progenitor cells along with a few rows of chondroblasts were initially prelabelled in vitro with [3H]thymidine and were subsequently chased and cultured for as long as eight days. Such explants underwent a process of tissue regeneration, as after three days in culture they reconstituted the original structure of the organ, thus resembling the in vivo appearance of neonatal mandibular condylar cartilage. Cellular proliferation with subsequent differentiation in the regenerating tissue was followed by means of quantitative autoradiography. Immediately after labelling, the autoradiography-positive grains were confined exclusively to progenitor cells. The latter revealed a substantial ability to proliferate in vitro, a fact that was manifested by a progressive increase in the labelling index along the course of the culture. The latter process was followed by cellular differentiation thereby obtaining hypertrophic chondrocytes. The increase in the rate of labelling index and in the total number of [3H]thymidine-labelled cells was significantly correlated with the overall growth of the regenerating explants.
新生ICR小鼠下颌髁突软骨的顶端部分，包含完整的祖细胞区以及几排成软骨细胞，最初在体外用[3H]胸腺嘧啶核苷进行预标记，随后进行追踪并培养长达8天。此类外植体经历了组织再生过程，因为在培养三天后它们重新构建了器官的原始结构，从而类似于新生下颌髁突软骨的体内外观。通过定量放射自显影追踪再生组织中的细胞增殖及随后的分化过程。标记后立即观察到，放射自显影阳性颗粒仅局限于祖细胞。后者显示出在体外具有强大的增殖能力，这一事实表现为随着培养进程标记指数逐渐增加。随后是细胞分化过程，从而获得肥大软骨细胞。标记指数率的增加以及[3H]胸腺嘧啶核苷标记细胞总数的增加与再生外植体的整体生长显著相关。
