Rune G M, Pretzer D, Beuthe W, Merker H J
Institut für Anatomie, Berlin, Federal Republic of Germany.
Cell Tissue Res. 1992 May;268(2):369-76. doi: 10.1007/BF00318805.
The influence of follicle-stimulating hormone, forskolin, insulin-like growth factor type I, epidermal growth factor, and 12-tetradecanoyl-phorbol-13-acetate on marmoset granulosa cell communication via gap junctions was investigated by morphological means and microinjection of carboxyfluorescein. Gap junctions between neighbouring granulosa cells were present in all groups. The number, but not length, of gap junctions between marmoset granulosa cells increased when the cells had been treated with follicle-stimulating hormone, insulin-like growth factor type I, and follicle-stimulating hormone plus insulin-like growth factor type I. No effect on gap junctions was seen, after exposure of the cells to the other three substances. Carboxyfluorescein and counting of the surrounding labelled cells showed that supplementation with follicle-stimulating hormone, forskolin, insulin-like growth factor type I and epidermal growth factor from the beginning of cultivation led to an increase in stained cells after 48 h. When treatment was started in 48 h cultures the substances reached their maximal activity within 30 min (forskolin and epidermal growth factor) or 3 h (follicle-stimulating hormone and insulin-like growth factor type I). Spreading of the fluorescent dye was inhibited when the medium was supplemented with 12-tetradecanoyl-phorbol-13-acetate. This effect was maximal after 30 min. Additive effects regarding the coupling of the cells were seen by combining of epidermal growth factor with follicle-stimulating hormone, but not with insulin-like growth factor type I or forskolin plus follicle-stimulating hormone.
通过形态学方法和羧基荧光素显微注射，研究了促卵泡激素、福斯高林、I型胰岛素样生长因子、表皮生长因子和12-十四酰佛波醇-13-乙酸酯对狨猴颗粒细胞通过缝隙连接进行细胞通讯的影响。所有组中相邻颗粒细胞之间均存在缝隙连接。当细胞用促卵泡激素、I型胰岛素样生长因子以及促卵泡激素加I型胰岛素样生长因子处理后，狨猴颗粒细胞之间缝隙连接的数量增加，但长度未增加。细胞暴露于其他三种物质后，缝隙连接未见影响。羧基荧光素和对周围标记细胞进行计数显示，从培养开始就补充促卵泡激素、福斯高林、I型胰岛素样生长因子和表皮生长因子，48小时后染色细胞数量增加。当在48小时培养物中开始处理时，这些物质在30分钟（福斯高林和表皮生长因子）或3小时（促卵泡激素和I型胰岛素样生长因子）内达到最大活性。当培养基中添加12-十四酰佛波醇-13-乙酸酯时，荧光染料的扩散受到抑制。这种作用在30分钟后达到最大。将表皮生长因子与促卵泡激素联合使用时，观察到细胞偶联方面的相加作用，但与I型胰岛素样生长因子或福斯高林加促卵泡激素联合使用时未观察到相加作用。
