Fitzgerald D J, Knowles S E, Ballard F J, Murray A W
Cancer Res. 1983 Aug;43(8):3614-8.
HEL-37 cells rapidly transferred microinjected fluorescein between contacting cells. This transfer was strongly inhibited by tumor-promoting phorbol esters and mezerein but not by non-promoting derivatives. The onset of the inhibition occurred after 5 to 10 min of exposure to the test compound and was complete within 20 min. Using a wash-off procedure, it was demonstrated that inhibition of dye transfer by phorbol-12, 13-dibutyrate was fully reversed by 100 min, after a lag of 40 to 60 min. The recovery of dye transfer competence was blocked by puromycin. Both 12-O-tetradecanoylphorbol-13-acetate and monensin caused elevated Na+ levels in HEL-37 cells, but the latter compound did not inhibit dye transfer. It was concluded that the promoter inhibition of transfer was not a consequence of Na+-induced increase in cytoplasmic Ca2+ concentration.
HEL-37细胞能在相互接触的细胞间迅速转移显微注射的荧光素。这种转移受到促肿瘤佛波酯和芫花酯甲的强烈抑制，但不受非促肿瘤衍生物的抑制。抑制作用在接触测试化合物5至10分钟后开始，并在20分钟内完全显现。采用洗脱程序表明，佛波醇-12,13-二丁酸酯对染料转移的抑制作用在滞后40至60分钟后，100分钟内可完全逆转。染料转移能力的恢复受到嘌呤霉素的阻断。12-O-十四酰佛波醇-13-乙酸酯和莫能菌素均可使HEL-37细胞内的钠离子水平升高，但后者并不抑制染料转移。由此得出结论，促肿瘤剂对转移的抑制并非由钠离子诱导的细胞质钙离子浓度升高所致。
