XIAP 5'非翻译区（UTR）内的异常剪接发生在海肾荧光素酶/萤火虫荧光素酶双顺反子报告系统中，而不在β-半乳糖苷酶/氯霉素乙酰转移酶双顺反子报告系统中。
Spurious splicing within the XIAP 5' UTR occurs in the Rluc/Fluc but not the betagal/CAT bicistronic reporter system.
作者信息
Holcik Martin, Graber Tyson, Lewis Stephen M, Lefebvre Charles A, Lacasse Eric, Baird Stephen
机构信息
Apoptosis Research Center, Children's Hospital of Eastern Ontario, Ottawa, Canada.
出版信息
RNA. 2005 Nov;11(11):1605-9. doi: 10.1261/rna.2158605. Epub 2005 Sep 21.
Abstract
X-chromosome-linked inhibitor of apoptosis, XIAP, has been shown to contain a strong internal ribosome entry site (IRES) within its 5' untranslated region (UTR) that promotes translation of XIAP mRNA under conditions of cellular stress. This claim came under scrutiny in a recent report demonstrating that the XIAP 5' UTR undergoes splicing when inserted between the two reporter cistrons of the dual luciferase plasmid Rluc/Fluc. In this paper, we demonstrate that the splicing within the XIAP 5' UTR specifically occurs only in the context of mRNA produced from the Rluc/Fluc but not the pbetagal/CAT bicistronic reporter plasmid.
摘要
X染色体连锁凋亡抑制蛋白（XIAP）已被证明在其5'非翻译区（UTR）内含有一个强大的内部核糖体进入位点（IRES），该位点在细胞应激条件下促进XIAP mRNA的翻译。最近的一份报告对这一说法进行了审查，该报告表明，当XIAP 5'UTR插入双荧光素酶质粒Rluc/Fluc的两个报告顺反子之间时会发生剪接。在本文中，我们证明XIAP 5'UTR内的剪接仅在Rluc/Fluc产生的mRNA背景下特异性发生，而不是在pbetagal/CAT双顺反子报告质粒产生的mRNA背景下发生。
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