Deventer K, Eenoo P Van, Delbeke F T
Ghent University (UGent), Department of Clinical Chemistry, Microbiology and Immunology, Doping Control Laboratory (DoCoLab), Technologiepark 30, B-9052 Zwijnaarde, Belgium.
Biomed Chromatogr. 2006 May;20(5):429-33. doi: 10.1002/bmc.579.
A fast and selective LC/MS/MS method for the screening of four anabolic steroids in human urine has been developed and validated. Liquid-liquid extraction with diethyl ether was applied after enzymatic hydrolysis. Analyses were performed on an ion trap mass spectrometer equipped with electrospray ionisation. MS/MS was applied for all compounds. The analytical run time was 11 min. The LOD for all compounds varied between 1 and 10 ng/mL. Left-over A samples, which were declared positive by GC/MS for the presence of 3'-hydroxystanozolol, were assessed using the described method.
已开发并验证了一种用于筛查人尿中四种合成代谢类固醇的快速、选择性液相色谱/串联质谱法。酶促水解后采用乙醚液液萃取。在配备电喷雾电离的离子阱质谱仪上进行分析。所有化合物均采用串联质谱法。分析运行时间为11分钟。所有化合物的检测限在1至10纳克/毫升之间。使用所述方法对气相色谱/质谱法判定为含有3'-羟基司坦唑醇而呈阳性的留存A样本进行了评估。
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