Deventer K, Delbeke F T
Ghent University, Doping Control Laboratory, Salisburylaan 133, B-9820 Merelbeke, Belgium.
Rapid Commun Mass Spectrom. 2003;17(18):2107-14. doi: 10.1002/rcm.1157.
A selective and sensitive method for the screening of nine corticosteroids in human urine has been validated. Analyses were performed using an ion trap instrument equipped with an electrospray ionisation (ESI) interface. All corticosteroids were separated in less than 20 min after liquid/liquid extraction with diethyl ether. The limit of detection for all substances was 4 ng/mL or lower. The method was applied to detect betamethasone after the intramuscular injection of Diprophos. Betamethasone could be detected for up to 12 days after administration. Validation of the chromatographic separation and mass spectrometric identification of mixtures of betamethasone and dexamethasone are also presented.
一种用于筛查人尿中九种皮质类固醇的选择性灵敏方法已得到验证。分析使用配备电喷雾电离（ESI）接口的离子阱仪器进行。在用乙醚进行液/液萃取后，所有皮质类固醇在不到20分钟内即可分离。所有物质的检测限为4 ng/mL或更低。该方法用于检测肌肉注射二丙磷后体内的倍他米松。给药后长达12天均可检测到倍他米松。文中还介绍了倍他米松和地塞米松混合物的色谱分离和质谱鉴定的验证情况。
