Pardo A G, Kemppainen M, Valdemoros D, Duplessis S, Martin F, Tagu D
Programa de Investigación en Interacciones Biológicas, Universidad Nacional de Quilmes, Roque Sáenz Peña 180, (B1876BXD) Bernal, Provincia de Buenos Aires, Argentina.
Rev Argent Microbiol. 2005 Apr-Jun;37(2):69-72.
The model ectomycorrhizal fungus Pisolithus microcarpus isolate 441 was transformed by using Agrobacterium tumefaciens LBA1100 and AGL-1. The selection marker was the Shble gene of Streptoallotecius hidustanus, conferring resistance to phleomycin, under the control of the gpd gene promoter and terminator of Schizophyllum commune. Transformation resulted in phleomycin resistant clones which were confirmed by PCR to contain the resistance cassette. A. tumefaciens-mediated gene transfer would allow the development of RNA interference technology in P. microcarpus.
利用根癌农杆菌LBA1100和AGL-1对模式外生菌根真菌微小皮氏多孔菌菌株441进行了转化。选择标记是嗜盐碱链霉菌的Shble基因，在裂褶菌gpd基因启动子和终止子的控制下赋予对腐草霉素的抗性。转化产生了对腐草霉素具有抗性的克隆，通过PCR确认其含有抗性盒。根癌农杆菌介导的基因转移将有助于在微小皮氏多孔菌中开发RNA干扰技术。
