Huang J F, Zhou X P, Cai J H, Li G X
Institute of Biotechnology, Zhejiang University, Hangzhou 310029, P.R. China.
Acta Virol. 2005;49(3):211-5.
Three begomovirus isolates, G46, G83 and G84 from Senecio scandens showing yellow mosaic symptoms were collected from Guangxi Province, P.R. China. The isolates were detected by PCR using universal primers for begomoviruses. Comparison of partial DNA-A sequences (approximately 500 bp) of the isolates revealed their 98.7-99.1% identity. The isolate G46, chosen for complete DNA-A sequencing, consisted of 2746 nt and had a typical genomic organization of begomoviruses. The G46 DNA-A had the highest sequence identity (72.4%) with that of Ageratum leaf curl virus among begomoviruses. The molecular data suggest that the isolate G46 is a new begomovirus (species), for which the name Senecio yellow mosaic virus (Senecio yellow mosaic virus) is proposed.
从中国广西省的千里光上采集到三株表现出黄色花叶症状的双生病毒分离物，分别为G46、G83和G84。使用双生病毒通用引物通过PCR对这些分离物进行检测。对分离物的部分DNA-A序列（约500 bp）进行比较，发现它们的同一性为98.7%-99.1%。选择分离物G46进行完整的DNA-A测序，其由2746个核苷酸组成，具有双生病毒典型的基因组结构。在双生病毒中，G46 DNA-A与藿香蓟曲叶病毒的序列同一性最高（72.4%）。分子数据表明，分离物G46是一种新的双生病毒（种），为此提出了千里光黄色花叶病毒（Senecio yellow mosaic virus）这一名称。
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