从中国菝葜中分离到的一种新型单分体曲叶病毒的分子特征。
Molecular characterization of a novel monopartite begomovirus isolated from Pouzolzia zeylanica in China.
机构信息
Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
出版信息
Arch Virol. 2013 Jul;158(7):1617-20. doi: 10.1007/s00705-013-1632-8. Epub 2013 Mar 5.
The complete genome sequence of a monopartite begomovirus isolate TY01 was obtained from diseased Pouzolzia zeylanica plants exhibiting golden mosaic symptoms in Baise, Guangxi Province, China. It consisted of 2723 nucleotides (nt) and encoded two ORFs (CP and AV2) in the virion-sense DNA and five ORFs (AC1-AC5) in the complementary-sense DNA. Compared with the DNA-A sequences of other begomoviruses, it has the highest (78.5 %) nucleotide sequence identity with ageratum yellow vein virus (AYVV) isolate AFSP6D from Thailand, which is less than the 89 % identity in the complete genome that has been defined as the threshold value for demarcation of species in the genus Begomovirus, family Geminiviridae. Phylogenetic analysis showed that TY01 was grouped in a separate clade from the other 28 begomovirus isolates. These results indicate that isolate TY01 is a member of a novel Begomovirus species, for which the name "Pouzolzia golden mosaic virus" (PGMV) is proposed.
从中国广西百色表现出黄色花叶症状的发病泡叶冷水花植株中获得了一个单分体番茄黄曲叶病毒（TY01）分离物的完整基因组序列。它由 2723 个核苷酸（nt）组成，在病毒 sense DNA 中编码两个 ORF（CP 和 AV2），在互补 sense DNA 中编码五个 ORF（AC1-AC5）。与其他番茄黄曲叶病毒的 DNA-A 序列相比，它与来自泰国的 Ageratum yellow vein virus（AYVV）分离物 AFSP6D 的核苷酸序列具有最高的同源性（78.5%），而在完整基因组中，这种同源性低于 89%，被定义为在双生病毒科 Geminiviridae 属中划分物种的阈值。系统发育分析表明，TY01 与其他 28 个番茄黄曲叶病毒分离物聚类在一个不同的分支中。这些结果表明，分离物 TY01 是一个新的番茄黄曲叶病毒物种的成员，建议将其命名为“泡叶冷水花黄花叶病毒”（PGMV）。
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