人胃肠道肥大细胞的细胞表面膜抗原表型

Cell surface membrane antigen phenotype of human gastrointestinal mast cells.

作者信息

Krauth Maria-Theresa, Majlesi Yasamin, Florian Stefan, Bohm Alexandra, Hauswirth Alexander W, Ghannadan Minoo, Wimazal Friedrich, Raderer Markus, Wrba Friedrich, Valent Peter

机构信息

Department of Internal Medicine I, Division of Hematology and Hemostaseology, Medical University of Vienna, Vienna, Austria.

出版信息

Int Arch Allergy Immunol. 2005 Oct;138(2):111-20. doi: 10.1159/000088432. Epub 2005 Sep 20.

DOI:10.1159/000088432

PMID:16179791

Abstract

BACKGROUND

Mast cells (MC) are important effector cells of allergic and inflammatory reactions in diverse organs. These cells interact with a number of other immune cells and structural cells in the tissues as well as with proinflammatory mediators and cytokines. The various interactions are considered to be mediated through distinct cell surface membrane receptors on MC.

METHODS

In the present study, we have established the cell surface membrane phenotype of human gastrointestinal MC (HGMC) using a panel of monoclonal antibodies and indirect immunofluorescence staining techniques.

RESULTS

HGMC were found to react with antibodies against CD29, CD33, CD44, CD45, CD47, CD54, CD55, CD58, CD63, CD117, CD147, CD151, CD172a, and CD203c. By contrast, HGMC did not express detectable amounts of CD1, CD2, CD4, CD5, CD14, CD15, CD16, CD22, CD24, CD25, CD26, CD27, CD28, CD31, CD32, CD34, CD35, CD88, or CD116. The alpha-chain of the IL-3 receptor (CD123) was detectable neither in resting HGMC nor in HGMC exposed to stem cell factor and interleukin-4.

CONCLUSIONS

HGMC express a unique profile of surface antigens including the receptor for mast cell growth factor, adhesion-related molecules, and activation-linked membrane antigens.

摘要

背景

肥大细胞（MC）是多种器官中过敏和炎症反应的重要效应细胞。这些细胞与组织中的许多其他免疫细胞和结构细胞相互作用，也与促炎介质和细胞因子相互作用。各种相互作用被认为是通过MC上不同的细胞表面膜受体介导的。

方法

在本研究中，我们使用一组单克隆抗体和间接免疫荧光染色技术建立了人胃肠道肥大细胞（HGMC）的细胞表面膜表型。

结果

发现HGMC与抗CD29、CD33、CD44、CD45、CD47、CD54、CD55、CD58、CD63、CD117、CD147、CD151、CD172a和CD203c的抗体发生反应。相比之下，HGMC未表达可检测量的CD1、CD2、CD4、CD5、CD14、CD15、CD16、CD22、CD24、CD25、CD26、CD27、CD28、CD31、CD32、CD34、CD35、CD88或CD116。IL-3受体（CD123）的α链在静息HGMC中以及暴露于干细胞因子和白细胞介素-4的HGMC中均未检测到。