Pérez T, Ercilla G, Chan W C, Haro I
Department of Peptide and Protein Chemistry, IIQAB-CSIC, Barcelona, Spain.
J Pept Sci. 2006 Apr;12(4):267-78. doi: 10.1002/psc.719.
In this work, new putative epitopes located in nonstructural proteins of GBV-C/HGV were synthesized using solid-phase chemistry for their use in immunoassays. The antigens were obtained in linear, chimeric and cyclic forms with the main aim of improving the sensitivity of the enzyme immunoassays. Our results showed, on one hand, that the combination of different antigens seems to be necessary to ensure good sensitivity and more specificity and, on the other hand, that cyclic compounds show higher ability to recognize anti-GBV-C/HGV antibodies than its parent peptide. Furthermore, CD and FTIR have been used in conjunction to characterize the conformational changes therein with synthetic constructs that could explain their different antigenicity.
在本研究中，利用固相化学合成了位于GBV-C/HGV非结构蛋白中的新的假定表位，用于免疫测定。以线性、嵌合和环状形式获得抗原，主要目的是提高酶免疫测定的灵敏度。我们的结果一方面表明，似乎需要不同抗原的组合来确保良好的灵敏度和更高的特异性，另一方面表明，环状化合物比其亲本肽表现出更高的识别抗GBV-C/HGV抗体的能力。此外，已结合使用圆二色光谱（CD）和傅里叶变换红外光谱（FTIR）来表征其中的构象变化以及能够解释其不同抗原性的合成构建体。
