精子发生过程中的生殖细胞凋亡控制
Germ cell apoptosis control during spermatogenesis.
作者信息
Giampietri Claudia, Petrungaro Simonetta, Coluccia Pierpaolo, D'Alessio Alessio, Starace Donatella, Riccioli Anna, Padula Fabrizio, Palombi Fioretta, Ziparo Elio, Filippini Antonio, De Cesaris Paola
机构信息
Department of Histology and Medical Embryology, Istituto Pasteur-Fondazione Cenci Bolognetti, University of Rome La Sapienza, 00161 Rome, Italy.
出版信息
Contraception. 2005 Oct;72(4):298-302. doi: 10.1016/j.contraception.2005.04.011.
The aim of the present study was to investigate the expression and role of c-Flip long isoform (c-FlipL), a known anti-apoptotic protein. No data are currently available on c-FlipL in male gonad before puberty; therefore, this study was carried out in prepuberal mouse testis. We investigated pachytene spermatocytes and spermatogonia by immunostaining of testis sections and found a strong and specific expression of c-FlipL in pachytene spermatocytes, while spermatogonia expressed very low levels of c-FlipL. This finding inversely correlated with the caspases activity, which was higher in spermatogonia as compared to pachytene spermatocytes. Other experiments carried out in an organ-culture model revealed that Fas-induced apoptosis was higher in spermatogonia as compared to pachytene spermatocytes. These data suggest that c-FlipL may play a role as an anti-apoptotic molecule in the prepuberal mouse testis and open new perspectives in the comprehension of the mechanisms controlling germ cells apoptosis.
本研究的目的是调查已知的抗凋亡蛋白c-FLIP长亚型（c-FlipL）的表达及其作用。目前尚无青春期前雄性性腺中c-FlipL的相关数据；因此，本研究在青春期前小鼠睾丸中进行。我们通过对睾丸切片进行免疫染色来研究粗线期精母细胞和精原细胞，发现c-FlipL在粗线期精母细胞中有强烈且特异性的表达，而精原细胞中c-FlipL的表达水平非常低。这一发现与半胱天冬酶活性呈负相关，与粗线期精母细胞相比，精原细胞中的半胱天冬酶活性更高。在器官培养模型中进行的其他实验表明，与粗线期精母细胞相比，精原细胞中Fas诱导的凋亡更高。这些数据表明，c-FlipL可能在青春期前小鼠睾丸中作为一种抗凋亡分子发挥作用，并为理解控制生殖细胞凋亡的机制开辟了新的视角。
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