Lord Megan S, Stenzel Martina H, Simmons Anne, Milthorpe Bruce K
Graduate School of Biomedical Engineering, The University of New South Wales, Sydney, NSW 2052 Australia.
Biomaterials. 2006 Mar;27(8):1341-5. doi: 10.1016/j.biomaterials.2005.09.007. Epub 2005 Sep 23.
Lysozyme interaction with an acrylic-based hydrogel, poly(2-hydroxyethyl methacrylate) co-methacrylic acid (P(HEMA-MAA)), was investigated using a combination of quartz crystal microbalance with dissipation (QCM-D), surface plasmon resonance (SPR) and dual polarisation interferometry (DPI). This combination of techniques demonstrated that lysozyme initially absorbed into the hydrogel matrix and displaced water from the hydrogel while subsequent lysozyme additions were adsorbed onto the surface of the hydrogel material. QCM-D, being sensitive to bound water, showed an overall decrease in mass and stiffening of the layer after lysozyme addition. SPR, a water insensitive technique, showed a net mass increase after addition of lysozyme and buffer rinses. DPI showed that the first exposure of lysozyme to P(HEMA-MAA) was consistent with lysozyme absorption while subsequent lysozyme exposures were consistent with lysozyme adsorption.
利用石英晶体微天平与耗散技术（QCM-D）、表面等离子体共振（SPR）和双偏振干涉测量法（DPI）相结合的方法，研究了溶菌酶与丙烯酸基水凝胶聚（甲基丙烯酸2-羟乙酯）-共-甲基丙烯酸（P(HEMA-MAA)）之间的相互作用。这些技术的组合表明，溶菌酶最初被吸收到水凝胶基质中，并将水从水凝胶中置换出来，而随后添加的溶菌酶则吸附在水凝胶材料的表面。对结合水敏感的QCM-D显示，添加溶菌酶后，质量总体下降，层变硬。SPR是一种对水不敏感的技术，添加溶菌酶并进行缓冲液冲洗后，显示净质量增加。DPI表明，溶菌酶首次暴露于P(HEMA-MAA)时与溶菌酶的吸收一致，而随后溶菌酶的暴露与溶菌酶的吸附一致。
