Drgon T, Sabová L, Gavurniková G, Kolarov J
Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
FEBS Lett. 1992 Jun 15;304(2-3):277-80. doi: 10.1016/0014-5793(92)80637-v.
Saccharomyces cerevisiae strains expressing a single type of ADP/ATP carrier (AAC) protein were prepared from a mutant in which all AAC genes were disrupted, by transformation with plasmids containing a chosen AAC gene. As demonstrated by measurements of [14C]ADP specific binding and transport, all three translocator proteins, AAC1, AAC2 and AAC3 when present in the mitochondrial membrane, exhibited similar translocation properties. The disruption of some AAC genes, however, resulted in phenotypes indicating that the function of these proteins in whole cells can be quite different. Specifically, we found that the disruption of AAC1 gene, but not AAC2 and AAC3, resulted in a change in colony phenotype.
通过用含有选定 AAC 基因的质粒转化，从所有 AAC 基因均被破坏的突变体中制备出表达单一类型 ADP/ATP 载体（AAC）蛋白的酿酒酵母菌株。如通过对[14C]ADP 特异性结合和转运的测量所表明的，当三种转运蛋白 AAC1、AAC2 和 AAC3 存在于线粒体膜中时，表现出相似的转运特性。然而，一些 AAC 基因的破坏导致了表型，表明这些蛋白在全细胞中的功能可能有很大差异。具体而言，我们发现破坏 AAC1 基因而非 AAC2 和 AAC3 会导致菌落表型发生变化。
