一起刑事案件中多次HIV-1传播的分子检测
Molecular testing of multiple HIV-1 transmissions in a criminal case.
作者信息
Lemey Philippe, Van Dooren Sonia, Van Laethem Kristel, Schrooten Yoeri, Derdelinckx Inge, Goubau Patrick, Brun-Vézinet Francoise, Vaira Dolores, Vandamme Anne-Mieke
机构信息
Rega Institute, KULeuven, B-3000 Leuven, Belgium.
出版信息
AIDS. 2005 Oct 14;19(15):1649-58. doi: 10.1097/01.aids.0000187904.02261.1a.
OBJECTIVE
To test the a priori hypothesis of HIV-1 transmission from one suspect to six recipients in a criminal case.
METHODS
Partial pol and/or env sequences were obtained for at least two samples of the suspect and the victims. Appropriate local controls were sampled based on epidemiological and subtype criteria. Phylogenetic testing was performed using different reconstruction methods.
RESULTS
Phylogenetic analyses consistently inferred a monophyletic cluster for the suspect and victim samples in both genome regions. This was highly supported by parametric and non-parametric bootstrapping techniques. Moreover, the controls most closely related to the suspect-victim cluster had a similar geographical origin to the suspect.
CONCLUSIONS
Taking into account the limitations on the conclusions that can be drawn from molecular investigations we could infer that our molecular data is consistent with a scenario of multiple HIV transmission between suspect and victims.
目的
在一宗刑事案件中检验关于人类免疫缺陷病毒1型（HIV-1）从一名嫌疑人传播至六名接受者的先验假设。
方法
获取嫌疑人及受害者至少两份样本的部分聚合酶（pol）和/或包膜（env）序列。根据流行病学和亚型标准采集适当的本地对照样本。使用不同的重建方法进行系统发育测试。
结果
系统发育分析始终推断出嫌疑人样本和受害者样本在两个基因组区域均形成一个单系簇。这得到了参数和非参数自展技术的高度支持。此外，与嫌疑人-受害者簇关系最密切的对照样本与嫌疑人具有相似的地理来源。
结论
考虑到分子调查所得结论存在的局限性，我们可以推断，我们的分子数据与嫌疑人与受害者之间多次发生HIV传播的情况相符。
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