Hamada Katsuyuki, Ueda Norifumi, Ito Masaharu, Roth Jack A, Follen Michele
Section of Thoracic Molecular Oncology, Department of Thoracic and Cardiovascular Surgery, The University of Texas M. D. Anderson Cancer Center, Houston, TX 77030, USA.
Gynecol Oncol. 2005 Dec;99(3 Suppl 1):S159-65. doi: 10.1016/j.ygyno.2005.07.073. Epub 2005 Sep 26.
The purposes of this study were to establish intracervical tumors of the nude rat as an orthotopic experimental model for human cervical cancer and to preliminary evaluate the effects of the adenoviral vector, Ad5CMV-p53, on orthotopic cervical tumor size.
Human cervical cancer SiHa and ME-180 cells were injected into the cervix of the nude rat. Four days later, 1 x 10(9) plaque forming units (PFU) of Ad5CMV-p53 were injected into the cervix. The rats were later sacrificed to determine cervical tumor size.
Eight of ten nude rats developed SiHa cell tumors; all ten nude rats developed ME-180 cell tumors. Four of ten SiHa cell tumors metastasized to the pelvic cavity; no ME-180 cell tumors did. The growth of Ad5CMV-p53-infected cells was greatly suppressed. The ad5CMV-p53 treatment significantly reduced both cell tumor volumes in nude rat cervixes.
The nude rat cervix grows tumors similar to human cervical cancer tumors and makes an excellent experimental model. Transfection of cervical cancer cells with the wild-type p53 gene via Ad5CMV-p53 is a potential therapeutic approach to cervical cancer.
本研究的目的是建立裸鼠宫颈原位肿瘤作为人类宫颈癌的原位实验模型，并初步评估腺病毒载体Ad5CMV-p53对原位宫颈肿瘤大小的影响。
将人宫颈癌SiHa和ME-180细胞注入裸鼠宫颈。4天后，将1×10⁹ 空斑形成单位（PFU）的Ad5CMV-p53注入宫颈。随后处死大鼠以确定宫颈肿瘤大小。
10只裸鼠中有8只发生SiHa细胞肿瘤；10只裸鼠均发生ME-180细胞肿瘤。10只SiHa细胞肿瘤中有4只转移至盆腔；ME-180细胞肿瘤均未转移。Ad5CMV-p53感染细胞的生长受到极大抑制。Ad5CMV-p53治疗显著减小了裸鼠宫颈中两种细胞肿瘤的体积。
裸鼠宫颈生长的肿瘤与人宫颈癌肿瘤相似，是一种优良的实验模型。通过Ad5CMV-p53将野生型p53基因转染至宫颈癌细胞是一种潜在的宫颈癌治疗方法。
