Analysis of the transcripts of cytochrome P-450IID gene subfamily in bovine adrenal gland.

An in situ hybridization experiment showed that the gene transcripts of P-450IID subfamily were uniformly distributed in bovine liver cells and at low levels in bovine adrenal cortex and medulla. There was no clear localization of transcripts of P-450IID genes in three adrenocortical zones, zona glomerulosa, zona fasciculata and zona reticularis. The intensity of signal obtained in the adrenal cortex and medulla showed approximately 2.5:1 ratio. The presence of gene transcripts of P-450IID subfamily in adrenocortex was confirmed by amplifying cDNA using PCR and hybridizing the PCR products with P-450IID cDNA.