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基于同步辐射的计算机显微断层扫描技术(SRICT)在特定生物矿物中的应用:胚胎期蜗牛、水母的平衡石以及人类牙齿。

Application of synchrotron-radiation-based computer microtomography (SRICT) to selected biominerals: embryonic snails, statoliths of medusae, and human teeth.

作者信息

Prymak Oleg, Tiemann Henry, Sötje Ilka, Marxen Julia C, Klocke Arndt, Kahl-Nieke Bärbel, Beckmann Felix, Donath Tilman, Epple Matthias

机构信息

Institute for Inorganic Chemistry, University Duisburg-Essen, Universitätsstrasse 5-7, 45117, Essen, Germany.

出版信息

J Biol Inorg Chem. 2005 Oct;10(6):688-95. doi: 10.1007/s00775-005-0023-3. Epub 2005 Nov 2.

Abstract

Synchrotron-radiation-based computer microtomography (SRmicroCT) was applied to three biomineralised objects First, embryonic snails of the freshwater snail Biomphalaria glabrata, second, rhopalia (complex sense organs) of the medusa Aurelia aurita, and third, human teeth. The high absorption contrast between the soft tissue and mineralised tissues, i.e. the shell in the first case (consisting of calcium carbonate) and the statoliths in the second case (consisting of calcium sulphate hemihydrate), makes this method ideal for the study of biomineralised tissues. The objects can be non-destructively studied on a micrometre scale, and quantitative parameters like the thickness of a forming a snail shell or statolith crystal sizes can be obtained on a length scale of 1-2 mum. Using SRmicroCT, the dentin-enamel border can be clearly identified in X-ray dense teeth.

摘要

基于同步辐射的计算机显微断层扫描技术(SRmicroCT)被应用于三个生物矿化物体。首先是淡水蜗牛光滑双脐螺的胚胎,其次是海月水母的缘膜(复杂感觉器官),第三是人类牙齿。软组织与矿化组织之间的高吸收对比度,即第一种情况下的外壳(由碳酸钙组成)和第二种情况下的平衡石(由半水硫酸钙组成),使得该方法成为研究生物矿化组织的理想选择。这些物体可以在微米尺度上进行无损研究,并且可以在1-2微米的长度尺度上获得诸如蜗牛壳形成的厚度或平衡石晶体尺寸等定量参数。使用SRmicroCT,可以在X射线致密的牙齿中清晰地识别牙本质-釉质边界。

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