Miller M W, Alevizos A, Cropper E C, Kupfermann I, Weiss K R
Center for Neurobiology and Behavior, New York State Psychiatric Institute, New York.
J Comp Neurol. 1992 Jun 8;320(2):182-95. doi: 10.1002/cne.903200204.
The neuropeptide buccalin A was originally purified and sequenced from a nerve-muscle system used in feeding-related behaviors of Aplysia californica in which it has been proposed that it acts as a modulatory cotransmitter. The distribution of buccalin-like immunoreactivity in the central ganglia and in peripheral tissues of Aplysia californica was examined by whole mount immunohistochemical techniques. Immunoreactive material was located in specific cell bodies and clusters of neurons in each of the ganglia. Immunoreactive fibers were present in each of the connectives between ganglia, in tracts coursing through the ganglia, and in the majority of the peripheral nerves. Most fibers were smooth in contour, but some had regularly spaced swellings. Varicosities containing immunoreactive material were located on specific neuronal somata and on certain tissues associated with the feeding, circulatory, digestive, and reproductive systems. The specific and widespread distribution of buccalin-like immunoreactivity supports the hypothesis that members of the buccalin peptide family act as neuromodulators or neurotransmitters in a variety of central and peripheral circuits in Aplysia.
神经肽颊肽A最初是从加州海兔与进食相关行为所使用的神经肌肉系统中纯化并测序得到的，有人提出它在该系统中作为一种调制性共递质发挥作用。采用整装免疫组织化学技术检测了颊肽样免疫反应性在加州海兔中枢神经节和外周组织中的分布。免疫反应性物质位于每个神经节中的特定细胞体和神经元簇中。免疫反应性纤维存在于神经节之间的每条连接中、穿过神经节的束中以及大多数外周神经中。大多数纤维轮廓光滑，但有些有规则间隔的膨体。含有免疫反应性物质的曲张体位于特定的神经元胞体以及与进食、循环、消化和生殖系统相关的某些组织上。颊肽样免疫反应性的特异性和广泛分布支持了这样一种假说，即颊肽家族成员在加州海兔的各种中枢和外周回路中作为神经调质或神经递质发挥作用。
