淋巴浆细胞淋巴瘤/华氏巨球蛋白血症的免疫表型特征

Immunophenotypic profile of lymphoplasmacytic lymphoma/Waldenström macroglobulinemia.

作者信息

Konoplev Sergej, Medeiros L Jeffrey, Bueso-Ramos Carlos E, Jorgensen Jeffrey L, Lin Pei

机构信息

Department of Hematopathology, The University of Texas M.D. Anderson Cancer Center, Houston 77030, USA.

出版信息

Am J Clin Pathol. 2005 Sep;124(3):414-20. doi: 10.1309/3G1X-DX0D-VHBN-VKB4.

DOI:10.1309/3G1X-DX0D-VHBN-VKB4

PMID:16191510

Abstract

We retrospectively reviewed the immunophenotypic profile of 75 cases of lymphoplasmacytic lymphoma/Waldenström macroglobulinemia (LPL/WM) analyzed by flow cytometry. All patients had monoclonal IgM (median, 2,100 mg/dL [21 g/L]) in serum and were considered clinically to have WM. The neoplastic cells, in all cases, expressed monoclonal immunoglobulin light chain (k, 55; l, 20) and CD19, and every case assessed was positive for CD20 (n=68) and CD52 (n=60). The results for other antigens assessed in decreasing frequency of positivity were as follows: surface IgM (26/28 [93%]), CD79b (11/13 [85%]), CD11c (13/16 [81%]), CD25 (5/7 [71%]), CD23 (17/28 [61%]), CD38 (24/50 [48%]), FMC7 (11/29 [38%]), CD22 (4/12 [33%]), CD5 (3/65 [5%]), and CD10 (1/38 [3%]). These results show that the immunophenotype of LPL/WM is variable and overlaps with other B-cell lymphoproliferative disorders. CD23, usually of dim intensity, and CD11c are expressed commonly in LPL/WM. Rare CD5+ and CD10+ cases of LPL/WM also exist.

摘要

我们回顾性分析了75例经流式细胞术检测的淋巴浆细胞淋巴瘤/华氏巨球蛋白血症（LPL/WM）的免疫表型特征。所有患者血清中均有单克隆IgM（中位数为2,100mg/dL[21g/L]），临床上均诊断为WM。所有病例中的肿瘤细胞均表达单克隆免疫球蛋白轻链（κ，55例；λ，20例）和CD19，且所有评估病例的CD20（n = 68）和CD52（n = 60）均为阳性。其他评估抗原的阳性频率由高到低依次为：表面IgM（26/28[93%]）、CD79b（11/13[85%]）、CD11c（13/16[81%]）、CD25（5/7[71%]）、CD23（17/28[61%]）、CD38（24/50[48%]）、FMC7（11/29[38%]）、CD22（4/12[33%]）、CD5（3/65[5%]）和CD10（1/38[3%]）。这些结果表明，LPL/WM的免疫表型具有多样性，且与其他B细胞淋巴增殖性疾病存在重叠。CD23通常呈弱表达，CD11c在LPL/WM中普遍表达。也存在罕见的CD5+和CD10+的LPL/WM病例。