Gervasi Francesco, Lo Verso Raffaella, Giambanco Caterina, Cardinale Giovanni, Tomaselli Carmela, Pagnucco Guido
Division of Hematology, Department of Oncology, A.R.NA.S. Civico-Benfratelli, G. Di Cristina e M. Ascoli, Palermo, Italy.
Ann N Y Acad Sci. 2004 Dec;1028:457-62. doi: 10.1196/annals.1322.054.
Multiparametric clinical flow cytometry has evolved from two-parameter quantitative assessment of lymphocytes to assessment of many qualitative parameters of suspensions obtained from bone marrow, peripheral blood, and lymph nodes for hematopathology. Nowadays, lymphoma immunophenotyping is a necessary complement to morphology and molecular parameters in the diagnosis and monitoring of human hematopoietic malignancies. The aim of the present study was to determine whether immunophenotypic differences could be used to distinguish between non-Hodgkin's B cell lymphoma (NHL-B) and the normal B cell subpopulation by assessing the variability in the patterns of expression of some lymphoid antigens (CD5, CD19, FMC7, CD23, CD20, CD79b, CD38, CD22, CD10, sIgkappa, sIglambda, mIgA, mIgG, mIgM, and mIgD) in specimens obtained from patients with NHL-B. We have studied peripheral blood samples, lymph node suspensions, and bone marrow specimens from 20 patients with malignant lymphoma and from controls without oncohematologic disease. Some patients showed stable patterns of antigen expression that remained unchanged over time and were consistent from one specimen to another. Other patients showed more variability in the pattern of antigen expression from different specimens. The two-way cluster analysis of antigens revealed three patterns of expression: (1) most cells in most cases positive (CD5, CD19, CD20, CD23, CD45); (2) most cells in most cases negative (CD10, mIgG, CD22, CD23,CD38); and (3) a mixed pattern with a variable number of positive cases and a variable percentage of positive cells in individual cases (CD22, CD38, CD79b, FMC7, mIgD, mIgM, mIgA, mIgG, sIgkappa, sIglambda). The expression of several antigens was strongly interdependent, even when antigens belonged to entirely different gene families. Such antigen pairs were CD19/CD45; CD19/CD79b; CD23/Igkappa; and CD45/CD79b. Our results suggest that different factors may determine the stability or the variability of such multiantigen expression, particularly the biology and function of the different antigens and the mechanisms of disease dissemination and progression.
多参数临床流式细胞术已从淋巴细胞的双参数定量评估发展到对从骨髓、外周血和淋巴结获取的用于血液病理学的悬浮液的许多定性参数进行评估。如今，淋巴瘤免疫表型分析是人类造血系统恶性肿瘤诊断和监测中形态学和分子参数的必要补充。本研究的目的是通过评估非霍奇金B细胞淋巴瘤（NHL - B）患者标本中某些淋巴样抗原（CD5、CD19、FMC7、CD23、CD20、CD79b、CD38、CD22、CD10、sIgκ、sIgλ、mIgA、mIgG、mIgM和mIgD）表达模式的变异性，确定免疫表型差异是否可用于区分非霍奇金B细胞淋巴瘤和正常B细胞亚群。我们研究了20例恶性淋巴瘤患者以及无血液肿瘤疾病的对照者的外周血样本、淋巴结悬浮液和骨髓标本。一些患者显示出抗原表达的稳定模式，随时间保持不变且在不同标本间一致。其他患者不同标本的抗原表达模式变异性更大。抗原的双向聚类分析揭示了三种表达模式：（1）大多数情况下大多数细胞呈阳性（CD5、CD19、CD20、CD23、CD45）；（2）大多数情况下大多数细胞呈阴性（CD10、mIgG、CD22、CD23、CD38）；（3）一种混合模式，个别病例中阳性病例数和阳性细胞百分比各不相同（CD22、CD38、CD79b、FMC7、mIgD、mIgM、mIgA、mIgG、sIgκ、sIgλ）。即使抗原属于完全不同的基因家族，几种抗原的表达也强烈相互依赖。这样的抗原对有CD19/CD45；CD19/CD79b；CD23/Igκ；以及CD45/CD79b。我们的结果表明，不同因素可能决定这种多抗原表达的稳定性或变异性，特别是不同抗原的生物学和功能以及疾病传播和进展的机制。
