Kirchner Michal, Matisová Eva, Hrouzková Svetlana, de Zeeuw Jaap
Department of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, Bratislava 812 37, Slovak Republic.
J Chromatogr A. 2005 Oct 7;1090(1-2):126-32. doi: 10.1016/j.chroma.2005.06.090.
In this work the application and limitations of a common bench top quadrupole mass spectrometer was evaluated for the qualitative and quantitative measurement of n-alkanes and pesticides of a wide range of volatilities and polarities with fast GC separations using 0.15 mm I.D. narrow-bore capillary columns. It was found that the spectra acquisition rate has a great impact on sensitivity (peak areas, peak shapes and S/N ratios). The quality of the obtained spectra is not significantly influenced in the full scan monitoring mode for the fastest scan rates. For quantitative analysis a selected ion monitoring mode is able to acquire the sufficient number of data-points for the proper peak shape reconstruction and good repeatability of peak areas measurements expressed by RSD (< 5%) for all tested dwell times shorter than 75 ms. However, for shorter dwell times, S/N ratio is lower, while peak areas are not influenced.
在这项工作中，使用内径0.15 mm的窄口径毛细管柱，通过快速气相色谱分离，评估了普通台式四极杆质谱仪在定性和定量测量具有广泛挥发性和极性的正构烷烃和农药方面的应用及局限性。结果发现，光谱采集速率对灵敏度（峰面积、峰形和信噪比）有很大影响。在全扫描监测模式下，对于最快的扫描速率，所获得光谱的质量受影响不显著。对于定量分析，在所有短于75 ms的测试驻留时间下，选择离子监测模式能够获取足够数量的数据点以进行适当的峰形重建，并且峰面积测量具有良好的重复性（相对标准偏差<5%）。然而，对于更短的驻留时间，信噪比更低，而峰面积不受影响。
