Li Dazhu, Ranjit Sharma, Zeng Qiutang
Institute of Cardiovascular Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
J Huazhong Univ Sci Technolog Med Sci. 2005;25(4):397-9, 415. doi: 10.1007/BF02828206.
To investigate the function of dendritic cells (DC) in patients with unstable angina, 10 mL of blood was drawn from 30 subjects. 15 patients diagnosed as having unstable angina and 15 healthy subjects were included in an observation and a control groups respectively. The mononuclear cells were separated from the peripheral blood and cultured in RPM11640 supplemented with recombinant human granulocyte/macrophage-colony stimulating factor (rh GM-CSF) and recombinant human interleukin-4 (rh IL-4) to induce dendritic cells. The shape and ultrastructure of DC was examined with electronic microscope. The phenotype of DC was analyzed with FACS and the alloantigen presenting capacity of DC was evaluated by mixed lymphocyte reaction (MLR). The expression rate of CD86 of DC in patients with unstable angina was (40.7 +/- 3.6) %, which was obviously higher than that of normal DC (29.6 +/- 2.5%) ( P < 0.001). The capacity of the DCs in unstable angina patients to induce allogenic T cells (OD 2.73 +/- 1.10), was significantly higher than that of the normal DC (OD:0.9 +/- 0.21) (P < 0.005). It is suggested that the function of DC in patients with unstable angina is increased, which may play an important role in the initiation of immune reaction in the plaque.
为研究树突状细胞（DC）在不稳定型心绞痛患者中的作用，从30名受试者中抽取10 mL血液。15例诊断为不稳定型心绞痛的患者和15名健康受试者分别纳入观察组和对照组。从外周血中分离出单核细胞，并在添加重组人粒细胞/巨噬细胞集落刺激因子（rh GM-CSF）和重组人白细胞介素-4（rh IL-4）的RPMI1640培养基中培养以诱导树突状细胞。用电子显微镜检查DC的形态和超微结构。用流式细胞术分析DC的表型，并用混合淋巴细胞反应（MLR）评估DC的同种异体抗原呈递能力。不稳定型心绞痛患者DC的CD86表达率为（40.7±3.6）%，明显高于正常DC的（29.6±2.5%）（P<0.001）。不稳定型心绞痛患者的DC诱导同种异体T细胞的能力（OD 2.73±1.10）明显高于正常DC（OD：0.9±0.21）（P<0.005）。提示不稳定型心绞痛患者DC功能增强，这可能在斑块免疫反应的启动中起重要作用。
