Chan Yoke-Fun, Abubakar Sazaly
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Malays J Pathol. 2003 Jun;25(1):29-35.
The effects of Enterovirus 71 (HEV71) infection on African green monkey kidney cells (Vero) were investigated. It was found that the infected cells showed progressive cellular morphological changes characteristic in apoptotic cells within 10 hours post-infection. The number of apoptotic cells correlated significantly with the number of HEV71 antigen positive cells when cells were labeled using terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) and stained for HEV71 antigen. Approximately 11, 26, 45 and 50% of the infected cells were apoptotic at 12, 24, 48 and 72 hours post-infection, respectively. Internucleosomal DNA fragmentation, characteristic in the late stage of apoptosis was noted beginning on day 2 post-infection. The DNA fragmentation, however, was absent in cells treated with the heat- and ultraviolet light-inactivated virus inocula. These results demonstrate the capacity of HEV71 to induce apoptosis in the infected cells. The induction, however, requires high level of HEV71 infectivity and the presence of live virus particles, suggesting the need for the presence of specific viral proteins for apoptosis to occur.
研究了肠道病毒71型（HEV71）感染对非洲绿猴肾细胞（Vero细胞）的影响。结果发现，感染细胞在感染后10小时内呈现出凋亡细胞特有的渐进性细胞形态变化。当使用末端脱氧核苷酸转移酶（TdT）介导的dUTP缺口末端标记（TUNEL）对细胞进行标记并对HEV71抗原进行染色时，凋亡细胞的数量与HEV71抗原阳性细胞的数量显著相关。在感染后12、24、48和72小时，分别约有11%、26%、45%和50%的感染细胞发生凋亡。在感染后第2天开始观察到凋亡后期特有的核小体间DNA片段化。然而，在用热灭活和紫外线灭活的病毒接种物处理的细胞中未观察到DNA片段化。这些结果证明了HEV71在感染细胞中诱导凋亡的能力。然而，这种诱导需要高水平的HEV71感染性和活病毒颗粒的存在，这表明凋亡的发生需要特定病毒蛋白的存在。
