Zhang Meixia, Yang Yang, Yan Mi, Zhang Junjun
West China Eye Center, West China Hospital, Sichuan University, 37 Guoxuexiang, Chengdu, Sichuan Province 610041, China.
Exp Eye Res. 2006 Jan;82(1):74-80. doi: 10.1016/j.exer.2005.05.005. Epub 2005 Sep 29.
Retinal neovascularization is among the leading causes of vision impairment throughout the world. Intraocular expression of vascular endothelial growth factor (VEGF), an angiogenic protein, and integrins, a group of cell adhesion molecules, is closely correlated with neovascularization in such neovascular diseases. The purpose of this study is to determine the effect of endostatin, a potent anti-angiogenic factor, on gene expression of vascular endothelial growth factor (VEGF) and integrinbeta3 in a mouse model of oxygen-induced retinopathy. C57BL/6 mice were given intravitreous injections of 1.0 microg endostatin at P12. At P17, retinal VEGF and integrinbeta3 mRNA levels were measured by real-time quantitative PCR in the hyperoxia mice and in the endostatin-treated mice. Analysis of 12 separate experiments revealed a 3.5-fold decrease in VEGF levels between hyperoxia mice and endostatin-treated mice (p<0.01) and a 2.5-fold decrease in integrinbeta3 levels between hyperoxia mice and endostatin-treated mice (p<0.01). These data suggest that intraocular expression of VEGF and integrinbeta3 mRNA is down-regulated by endostatin, which may provide a new therapeutic approach for ocular neovascularization.
视网膜新生血管形成是全球视力损害的主要原因之一。血管内皮生长因子（VEGF，一种血管生成蛋白）和整合素（一组细胞粘附分子）的眼内表达与这类新生血管疾病中的新生血管形成密切相关。本研究的目的是在氧诱导性视网膜病变小鼠模型中确定内皮抑素（一种有效的抗血管生成因子）对血管内皮生长因子（VEGF）和整合素β3基因表达的影响。在出生后第12天给C57BL/6小鼠玻璃体内注射1.0微克内皮抑素。在出生后第17天，通过实时定量PCR测量高氧小鼠和内皮抑素治疗小鼠的视网膜VEGF和整合素β3 mRNA水平。对12个独立实验的分析显示，高氧小鼠和内皮抑素治疗小鼠之间VEGF水平降低了3.5倍（p<0.01），高氧小鼠和内皮抑素治疗小鼠之间整合素β3水平降低了2.5倍（p<0.01）。这些数据表明，内皮抑素可下调VEGF和整合素β3 mRNA的眼内表达，这可能为眼内新生血管形成提供一种新的治疗方法。
