Lair D, Alexandre S, Valleton J M
UMR 6522, CNRS, Université de Rouen, 76821 Mont-Saint-Aignan, France.
Colloids Surf B Biointerfaces. 2005 Nov 10;45(3-4):200-8. doi: 10.1016/j.colsurfb.2005.08.012. Epub 2005 Sep 29.
The structure and the dynamic organization of a mixed Langmuir film of glucose oxidase and stearylamine at the air-water interface have been studied. The film has been first characterized at the air-water interface by surface pressure/area isotherms. The dynamics of the mixed film was studied by following the evolution of the film area at a constant pressure and the evolution of the pressure at a constant area. After transfer of the films on solid substrates, the chemical composition of the mixed film has been quantified by UV-vis and IR spectroscopies. These characterizations were carried out in order to study the incorporation of glucose oxidase into the stearylamine film, and its influence on the structural evolution of the film. From these results, the dynamic organization of this mixed film may be described. For short times, glucose oxidase molecules interact with stearylamine molecules in solution or at the interface; these interactions would lead to the formation of a complex between stearylamine and glucose oxidase molecules. For long times (at least 3 h), a homogeneous mixed film constituted essentially of this complex is obtained at the air-water interface. A detailed analysis by atomic force microscopy allowed us to support this model and the existence of the glucose oxidase/stearylamine complex.
研究了葡萄糖氧化酶和硬脂胺在空气-水界面形成的混合朗缪尔膜的结构和动态组织。该膜首先通过表面压力/面积等温线在空气-水界面进行表征。通过跟踪恒定压力下膜面积的变化以及恒定面积下压力的变化来研究混合膜的动力学。将膜转移到固体基质上后，通过紫外-可见光谱和红外光谱对混合膜的化学成分进行了定量。进行这些表征是为了研究葡萄糖氧化酶在硬脂胺膜中的掺入及其对膜结构演变的影响。根据这些结果，可以描述这种混合膜的动态组织。在短时间内，葡萄糖氧化酶分子与溶液中或界面处的硬脂胺分子相互作用；这些相互作用将导致硬脂胺和葡萄糖氧化酶分子之间形成复合物。在长时间（至少3小时）内，在空气-水界面获得了基本上由这种复合物构成的均匀混合膜。通过原子力显微镜进行的详细分析使我们能够支持该模型以及葡萄糖氧化酶/硬脂胺复合物的存在。
