Huang Qiaoyun, Liang Wei, Cai Peng
State Key Laboratory of Agricultural Microbiology, Faculty of Resources and Environment, Huazhong Agriculture University, Wuhan 430070, China.
Colloids Surf B Biointerfaces. 2005 Nov 10;45(3-4):209-14. doi: 10.1016/j.colsurfb.2005.08.011. Epub 2005 Sep 29.
Adsorption, desorption and activity of acid phosphatase on various soil colloidal particles and pure clay minerals were studied. Higher adsorption amounts and low percentage of desorption of acid phosphatase were found on fine soil clays (<0.2 microm). Electrostatic force and ligand exchange are the major driving forces that are involved in the adsorption of enzymes on soil clays. More enzyme molecules were adsorbed on soil clays in the presence of organic components. However, enzymes on organic clays were more easily released. One-third of the enzyme on goethite was adsorbed via ligand exchange process. Some other interactions, such as van der Waals force, hydrophobic force and hydrogen bonding may be more important in the adsorption of enzyme on kaolinite and the enzyme in this system cannot be easily removed. Coarse clays (0.2-2 microm) and inorganic soil clays had higher affinities for enzyme molecules than fine clays and organic clays, respectively. The activity of enzyme bound on soil clays was inhibited and the thermal stability was increased in the presence of organic matter. Data obtained in this study are helpful for a better understanding of the interactions of enzymes with inorganic and organic constituents in soil and associated environments.
研究了酸性磷酸酶在各种土壤胶体颗粒和纯粘土矿物上的吸附、解吸及活性。在细粒土壤粘土（<0.2微米）上发现酸性磷酸酶的吸附量较高且解吸百分比低。静电力和配体交换是酶吸附在土壤粘土上的主要驱动力。在有机成分存在下，更多的酶分子吸附在土壤粘土上。然而，有机粘土上的酶更容易释放。针铁矿上三分之一的酶通过配体交换过程吸附。一些其他相互作用，如范德华力、疏水力和氢键，在酶吸附在高岭石上可能更重要，并且该体系中的酶不易去除。粗粒粘土（0.2 - 2微米）和无机土壤粘土分别对酶分子的亲和力高于细粒粘土和有机粘土。在有机物存在下，结合在土壤粘土上的酶的活性受到抑制，热稳定性增加。本研究获得的数据有助于更好地理解酶与土壤及相关环境中无机和有机成分的相互作用。
