[甲烷同化酵母的NAD特异性脱氢酶催化甲醇氧化反应]
[Methanol oxidation by the NAD-specific dehydrogenase of methane-assimilating yeasts].
作者信息
Simisker Ia A, Mikelsaar S K, Heinara E H
出版信息
Mikrobiologiia. 1977 Jan-Feb;46(1):169-71.
The activity of NAD-specific dehydrogenase (NAD-DH) capable of methanol oxidation was found in cell-free extracts of methanol assimilating yeast cultures Pichia pinus and Candida boidinii. The optimum pH of the incubation mixture for the enzyme is 7.8--7.9; the value of Km of the enzyme for methanol is 1.4-10(-4) M in P. pinus and 1.04-10(-3) M in C. boidinii. NAD-DH is absent from the cells cultivated on glucose, and is induced with growth on a medium containing methanol.
在能够同化甲醇的酵母培养物毕赤酵母（Pichia pinus）和博伊丁假丝酵母（Candida boidinii）的无细胞提取物中，发现了能够氧化甲醇的NAD特异性脱氢酶（NAD-DH）的活性。该酶孵育混合物的最适pH值为7.8-7.9；在毕赤酵母中该酶对甲醇的Km值为1.4×10⁻⁴ M，在博伊丁假丝酵母中为1.04×10⁻³ M。在以葡萄糖培养的细胞中不存在NAD-DH，而在含有甲醇的培养基上生长时会被诱导产生。
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