[1,25-二羟维生素D3诱导HL-60细胞分化及nm23基因表达下调]
[Cell differentiation and down regulation of nm23 gene expression in HL-60 cells induced by 1,25 dihydroxyvitamin D3].
作者信息
Zhu N, Lu Q, Zhou Y
机构信息
Deparnent of Hematology , Zhejiang Provincial Hospital of Traditional Chinese Medicine, Hangzhou 310006, China.
出版信息
Zhonghua Xue Ye Xue Za Zhi. 2001 Dec;22(12):639-41.
OBJECTIVE
To investigate the differentiation induction effect of 1,25 dihydroxyvitamin D3 on leukemia cell line HL-60 and its mechanisms.
METHODS
The changes of proliferation rate and differentiation in HL-60 cells treated with 1,25 dihydroxyvitamin D3 were observed by MTT test, surface marker analysis and NBT reduction reaction, the effects on cell cycle and nm23 gene expression by flow cytometry and RT-PCR.
RESULTS
After exposing to 1,25 dihydroxyvitamin D3, the proliferation was inhibited and the cells were induced to differentiate towards more mature monocytes. During differentiation, HL-60 cells were arrested in G1 phase and the expression of nm23 gene was down regulated.
CONCLUSION
1,25 dihydroxyvitamin D3 could induce differentiation of HL-60 cells, this effect might be related to the down regulation of nm23 gene expression.
目的
探讨1,25-二羟维生素D3对白血病细胞株HL-60的诱导分化作用及其机制。
方法
采用MTT法、表面标志物分析及NBT还原反应观察1,25-二羟维生素D3处理后HL-60细胞增殖率及分化的变化，应用流式细胞术及RT-PCR检测其对细胞周期及nm23基因表达的影响。
结果
经1,25-二羟维生素D3作用后，HL-60细胞增殖受到抑制，并被诱导分化为更成熟的单核细胞。在分化过程中，HL-60细胞被阻滞于G1期，nm23基因表达下调。
结论
1,25-二羟维生素D3可诱导HL-60细胞分化，这一作用可能与nm23基因表达下调有关。
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