Liang P, Li Q, Liu E
Department of Neurosurgery, First Clincal College, Harbin Medical University, Harbin 150001, China.
Zhonghua Yi Xue Za Zhi. 2001 Nov 10;81(21):1324-6.
To isolate neural precursor cells from human embryonic cortical tissue of 20 weeks and identify their proliferation capacity as well as differentiating potential.
Human fetuses of about 20 weeks from spontaneous abortion were adopted. A serum-free medium containing basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) was used to make the neural precursor cell divide continuously in the culture. The proliferating ability of these cells was tested with BrdU. Growth factors were removed so as to induce differentiation of the precursor cells. The cell cycle of the cells in the neurosphere was analysed using flow cytometry.
Cells from human embryonic cortical tissue could be maintained and propagated in the presence of growth factors. Neurospheres generated continually and the cells in the sphere could incorporate into BrdU. Upon differentiation, the precursor cells gave rise to mature neurons, astrocytes and oligodendrocytes. They retained their multilineage potential over repeated passages. The cell cycle analysis indicated that the cells proliferated actively.
Cells that able to self-renew and differentiate into mature cells in culture cam be isolated from human embryonic tissues. They are verified to be neural precursor cells.
从20周龄的人类胚胎皮质组织中分离神经前体细胞，并鉴定其增殖能力和分化潜能。
采用自然流产的约20周龄人类胎儿。使用含有碱性成纤维细胞生长因子（bFGF）和表皮生长因子（EGF）的无血清培养基，使神经前体细胞在培养中持续分裂。用BrdU检测这些细胞的增殖能力。去除生长因子以诱导前体细胞分化。使用流式细胞术分析神经球中细胞的细胞周期。
在生长因子存在的情况下，人类胚胎皮质组织的细胞能够维持并增殖。持续产生神经球，球内细胞可掺入BrdU。分化后，前体细胞产生成熟神经元、星形胶质细胞和少突胶质细胞。它们在多次传代后仍保留其多系分化潜能。细胞周期分析表明细胞增殖活跃。
能够在培养中自我更新并分化为成熟细胞的细胞可从人类胚胎组织中分离出来。它们被证实为神经前体细胞。
