Ren M, Zhao J, Li H
Department of Obstetrics and Gynecology, Affiliated Hospital of Southeast University, Nanjing 210009, China.
Zhonghua Yi Xue Za Zhi. 2001 Dec 25;81(24):1516-8.
To study if infection of new type pathogenic mycoplasmas, Mycoplasma penetrans (Mpe), Mycoplasma fermentans (Mf), and Mycoplasma pirum (Mpi), exists in the genital tract of gynecologic patients with genital infection in China so as to provide information for clinical diagnosis and therapy.
Cervical secretions from 172 patients with infectious diaseses of gentital tract and 172 healthy controls were collected and inoculated in improved SP-4 culture medium. Biochemical reaction, coagglutination test, metabolism-inhibition test, polymerase chain reaction (PCR) assay, and DNA sequencing were employed to identify the isolated microorganisms.
One strain of Mycoplasma penetrans (0.58%) and two strains of Mycoplasma fermentans (1.16%) were isolated from the cervical secretions of patients, but none was found in healthy controls.
Mpe infection and Mf infection exist in female patients with genital infection in Nanjing.
研究新型致病支原体穿透支原体（Mpe）、发酵支原体（Mf）和梨支原体（Mpi）在中国妇科生殖器感染患者生殖道中是否存在，为临床诊断和治疗提供依据。
收集172例生殖道感染患者和172例健康对照者的宫颈分泌物，接种于改良SP-4培养基。采用生化反应、协同凝集试验、代谢抑制试验、聚合酶链反应（PCR）检测及DNA测序等方法对分离出的微生物进行鉴定。
从患者宫颈分泌物中分离出1株穿透支原体（0.58%）和2株发酵支原体（1.16%），健康对照者中未分离出。
南京地区女性生殖器感染患者中存在Mpe感染和Mf感染。
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