Shen Feng, Zhao Ming-wu, He Bei, Yang Jing-jing, Pei Fei, Wang Yu-zhu
Department of Respiratory Medicine, First Hospital, Peking University, Beijing, China.
Zhonghua Jie He He Hu Xi Za Zhi. 2004 Oct;27(10):654-8.
To investigate the changes of interleukin-17 (IL-17) both in rat models of chronic obstructive pulmonary disease (COPD) and in asthma.
Male SD rats were randomly divided into COPD group, asthma group, smoking group and control group. The concentrations of IL-17 in lung tissues and in bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immunosorbent assay (ELISA). The expression of IL-17 in lung tissue was determined by immunohistochemical staining and photographic analysis.
The concentrations of IL-17 both in the lung tissue and in the BALF were significantly higher in the COPD group and the asthma group than in the controls and the smoking group (all P <0. 01). IL-17 was mainly expressed in airway epithelial cells of COPD rats and in T lymphocytes around the airway in rats with asthma. The levels of IL-17 were also higher both in the COPD group and in the asthma group than in the smoking group and the controls (all P < 0. 01) evaluated by immunohistochemical staining and photographic analysis. In the COPD group,IL-17 concentration of lung tissue was positively correlated with percentage of neutrophils in BALF; it also tended to be positively correlated with the level of intercellular adhesion molecule-1 (ICAM-1) in airway epithelial cells, and the concentration of IL-17 in BALF was positively correlated with the degree of smooth muscle proliferation as well. The concentration of IL-17 in the lung tissue of the asthma group was positively correlated with the number of eosinophils in BALF and with the number of CD3 T lymphocytes in lung tissues respectively.
The production of IL-17 in COPD and in asthma might be distinct. IL-17 probably recruits neutrophils into airways by enhancing the expression of ICAM-1 in airway epithelial cells, and it might play a role in pathological changes of small airways in COPD. The role of IL-17 in asthma maybe associated with the recruitment of eosinophils into airways.
研究白细胞介素-17（IL-17）在慢性阻塞性肺疾病（COPD）大鼠模型和哮喘大鼠模型中的变化。
雄性SD大鼠随机分为COPD组、哮喘组、吸烟组和对照组。采用酶联免疫吸附测定（ELISA）法检测肺组织和支气管肺泡灌洗液（BALF）中IL-17的浓度。通过免疫组织化学染色和图像分析测定肺组织中IL-17的表达。
COPD组和哮喘组肺组织及BALF中IL-17浓度均显著高于对照组和吸烟组（均P<0.01）。IL-17主要表达于COPD大鼠的气道上皮细胞及哮喘大鼠气道周围的T淋巴细胞中。通过免疫组织化学染色和图像分析评估，COPD组和哮喘组IL-17水平也高于吸烟组和对照组（均P<0.01）。在COPD组，肺组织IL-17浓度与BALF中中性粒细胞百分比呈正相关；与气道上皮细胞中细胞间黏附分子-1（ICAM-1）水平也呈正相关趋势，且BALF中IL-17浓度与平滑肌增殖程度也呈正相关。哮喘组肺组织中IL-17浓度分别与BALF中嗜酸性粒细胞数量及肺组织中CD3 T淋巴细胞数量呈正相关。
COPD和哮喘中IL-17的产生可能不同。IL-17可能通过增强气道上皮细胞中ICAM-1的表达将中性粒细胞募集到气道中，并且可能在COPD小气道的病理变化中起作用。IL-17在哮喘中的作用可能与嗜酸性粒细胞向气道的募集有关。
