Wu Bin, Liu Jun-lin, Chen Min, Deng Ri-qiang, Wu Dong
Department of Respiratory Medicine, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524000, China.
Zhonghua Jie He He Hu Xi Za Zhi. 2004 Oct;27(10):668-71.
To investigate the correlation of - 1112C/T polymorphism of Interleukin-13 (IL-13) gene promoter with asthma and its effect on total plasma IgE levels.
One hundred asthmatic patients (the asthma group) and 100 healthy controls (the control group) were recruited. Polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP) techniques were used to determine the - 1112 locus polymorphism, and the total plasma IgE levels were determined by ELISA.
There were significant differences in C and T allele frequencies at -1112 locus (chi2 =9.01, P< 0.01), and in the genotype distribution such as CC, CT and TT between the two groups [chi2 =7.19, P < 0.05; OR(TT/CC) =2.99, 95%CI=1.06-8.41 (P<0.05); OR (CT/CC) =2.04, 95%CI=1.09-3.81 (P <0.05); OR(TT/CT) =1.46, 95% CI =0.49-4.37, P >0.05]. Allele T was associated with asthma, OR(T/C) = 2. 03, 95% CI =1.27-3.23 (P <0. 01). In the asthma group, the total plasma IgE levels of the patients with CC, CT and TT genotypes were (204 +/- 89)kU/L, (320 +/- 108)kU/L and (376 +/- 147) kU/L, respectively. While in the control group, the total plasma IgE levels of the subjects with CC, CT and TT genotypes were (96 +/- 34) kU/L, (122 +/- 42) kU/L and (150 +/- 36) kU/L, respectively. In both groups, the total plasma IgE levels in allele T carriers were higher than those in non- allele T carriers, and the total plasma IgE levels in the asthma group were higher than those in the control group with the same genotype.
The - 1112 locus polymorphism of IL-13 gene promoter may be an important candidate gene for asthma Allele T is associated with asthma and increases the total plasma IgE level via enhanced (enhancing) IL-13 gene expression.
探讨白细胞介素 - 13（IL - 13）基因启动子 - 1112C/T多态性与哮喘的相关性及其对血浆总IgE水平的影响。
招募100例哮喘患者（哮喘组）和100例健康对照者（对照组）。采用聚合酶链反应/限制性片段长度多态性（PCR/RFLP）技术检测 - 1112位点多态性，并用ELISA法测定血浆总IgE水平。
两组间 - 1112位点C和T等位基因频率有显著差异（χ² = 9.01，P < 0.01），基因型分布如CC、CT和TT也有差异[χ² = 7.19，P < 0.05；OR（TT/CC） = 2.99，95%CI = 1.06 - 8.41（P < 0.05）；OR（CT/CC） = 2.04，95%CI = 1.09 - 3.81（P < 0.05）；OR（TT/CT） = 1.46，95%CI = 0.49 - 4.37，P > 0.05]。等位基因T与哮喘相关，OR（T/C） = 2.03，95%CI = 1.27 - 3.23（P < 0.01）。在哮喘组中，CC、CT和TT基因型患者的血浆总IgE水平分别为（204 ± 89）kU/L、（320 ± 108）kU/L和（376 ± 147）kU/L。而在对照组中，CC、CT和TT基因型受试者的血浆总IgE水平分别为（96 ± 34）kU/L、（122 ± 42）kU/L和（150 ± 36）kU/L。两组中，等位基因T携带者的血浆总IgE水平均高于非等位基因T携带者，且哮喘组中相同基因型的血浆总IgE水平高于对照组。
IL - 13基因启动子 - 1112位点多态性可能是哮喘的重要候选基因。等位基因T与哮喘相关，并通过增强IL - 13基因表达增加血浆总IgE水平。
