Bahcecioglu Ibrahim Halil, Yalniz Mehmet, Ataseven Huseyin, Ilhan Necip, Ozercan Ibrahim Hanifi, Seckin Dilara, Sahin Kazim
Division of Gastroenterology, Firat University, Elazig, Turkey.
Hepatogastroenterology. 2005 Sep-Oct;52(65):1549-53.
BACKGROUND/AIMS: Nonalcoholic steatohepatitis (NASH) is a common cause of liver disease that comprises a wide spectrum of liver damage, ranging from simple steatosis to steatohepatitis. The aim of this study is to investigate serum hyaluronic acid (HA), TNF-alpha, IL-8 levels in patients with non-alcoholic steatohepatitis and to assess their potential value as a noninvasive marker for the severity of histopathology.
Twenty-eight patients with biopsy-proven NASH, 14 patients with cirrhosis and 15 healthy controls were studied. Histopathological findings were graded and staged. HA, IL-8, TNF-levels were determined using by ELISA test
Serum HA levels in patients with NASH were significantly higher than in the healthy control group (P < 0.05). However, the levels in patients with cirrhosis were markedly higher than in patients with NASH and healthy controls (P < 0.001). Serum TNF-alpha levels were significantly higher in patients with NASH and cirrhosis than in healthy controls (P < 0.05). Serum IL-8 levels in patients with NASH (P < 0.001) and cirrhosis (P < 0.05) were significantly higher than in the healthy control group. There was no correlation between serum HA and IL-8, TNF-alpha, ALT and AST levels. Serum HA level in patients with NASH was 187.26 +/- 139.21 and 143.49 +/- 93.14 in stage in stage 2-3 and in stage 0-1, respectively, but the difference was not significant (P > 0.05).
In conclusion, serum HA, IL-8 and TNF-alpha levels increased in patients with NASH. Their relation with the severity of histopathology is not significant. Serum HA levels may be a useful marker to monitor the conversion from fibrosis to cirrhosis. Further studies are needed on this topic.
背景/目的：非酒精性脂肪性肝炎（NASH）是肝脏疾病的常见病因，涵盖了从单纯性脂肪变性到脂肪性肝炎的广泛肝损伤范围。本研究旨在调查非酒精性脂肪性肝炎患者血清透明质酸（HA）、肿瘤坏死因子-α（TNF-α）、白细胞介素-8（IL-8）水平，并评估其作为组织病理学严重程度无创标志物的潜在价值。
对28例经活检证实的NASH患者、14例肝硬化患者和15名健康对照者进行研究。对组织病理学结果进行分级和分期。采用酶联免疫吸附测定（ELISA）法测定HA、IL-8、TNF水平。
NASH患者血清HA水平显著高于健康对照组（P<0.05）。然而，肝硬化患者的水平明显高于NASH患者和健康对照组（P<0.001）。NASH和肝硬化患者血清TNF-α水平显著高于健康对照组（P<0.05）。NASH患者（P<0.001）和肝硬化患者（P<0.05）血清IL-8水平显著高于健康对照组。血清HA与IL-8、TNF-α、谷丙转氨酶（ALT）和谷草转氨酶（AST）水平之间无相关性。NASH患者2-3期血清HA水平为187.26±139.21，0-1期为143.49±93.14，但差异无统计学意义（P>0.05）。
总之，NASH患者血清HA、IL-8和TNF-α水平升高。它们与组织病理学严重程度的关系不显著。血清HA水平可能是监测纤维化向肝硬化转变的有用标志物。关于该主题需要进一步研究。
