Frydrych Barbara, Szymańska Jadwiga A, Sala Monika, Bruchajzer Elzbieta
Depertment of Toxicology, Faculty of Pharmacy, Medical University, Lódź, Poland.
Int J Occup Med Environ Health. 2005;18(2):127-32.
Glutathione (GSH) is an important element of antioxidative barrier. Its biological function consists in eliminating oxygen free radicals. It also acts as a co-substrate in numerous enzymatic reactions catalyzed by glutathione peroxidase (GPx) and glutathione S-transferase (GST). In our study we attempted to assess the effect of hexabromobenzene (HBB) and its metabolites on the level of GSH and related enzymes, GPx and GST.
The experiments were performed on female Wistar rats. The investigated compounds (HBB, 1,2,4,5-tetraBB, 1,2,4- and 1,3,5-triBB) were administered intragastrically in three different doses (HBB: 15, 75, and 375 mg/kg; 1,2,4,5-tetraBB and 1,2,4-triBB: 8, 40, and 200 mg/kg; 1,3,5-triBB: 12, 60, and 300 mg/kg) for 7, 14, 21 or 28 days. GSH level and activity of GST and GPx were determined in the obtained material.
The highest activity of GPx and GSTwas observed after a 7-fold administration of all investigated compounds. Prolonged time of exposure caused the return to the control values.
The study revealed that repeated exposure to aromatic bromine derivatives increases GPx and GST activity only in the initial phase of the experiment.
谷胱甘肽（GSH）是抗氧化屏障的重要组成部分。其生物学功能在于清除氧自由基。它还在由谷胱甘肽过氧化物酶（GPx）和谷胱甘肽S-转移酶（GST）催化的众多酶促反应中作为共底物发挥作用。在我们的研究中，我们试图评估六溴苯（HBB）及其代谢产物对GSH水平以及相关酶GPx和GST的影响。
实验在雌性Wistar大鼠身上进行。将所研究的化合物（HBB、1,2,4,5-四溴苯、1,2,4-三溴苯和1,3,5-三溴苯）以三种不同剂量经胃内给药（HBB：15、75和375mg/kg；1,2,4,5-四溴苯和1,2,4-三溴苯：8、40和200mg/kg；1,3,5-三溴苯：12、60和300mg/kg），持续7、14、21或28天。在所获得的材料中测定GSH水平以及GST和GPx的活性。
在所有所研究的化合物给药7天后观察到GPx和GST的最高活性。延长暴露时间导致其活性恢复到对照值。
该研究表明，反复接触芳香族溴衍生物仅在实验初期会增加GPx和GST的活性。
